Oncotarget

Research Papers:

Expression pattern of genome-scale long noncoding RNA following acute myocardial infarction in Chinese Uyghur patients

Hui Zhai, Xiao-Mei Li, Fen Liu, Bang-Dang Chen, Hong Zheng, Xue-Mei Wang, Wu Liao, Qing-Jie Chen, Yi-Tong Ma and Yi-Ning Yang _

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Oncotarget. 2017; 8:31449-31464. https://doi.org/10.18632/oncotarget.16355

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Abstract

Hui Zhai1,2,*, Xiao-Mei Li1,2,*, Fen Liu2, Bang-Dang Chen2, Hong Zheng3, Xue-Mei Wang2, Wu Liao1,2, Qing-Jie Chen1,2, Yi-Tong Ma1,2, Yi-Ning Yang1,2

1Department of Cardiology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, China

2Xinjiang Key Laboratory of Cardiovascular Disease Research, Urumqi, China

3Anesthesia Department, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, China

*These authors contributed equally to this work

Correspondence to:

Yi-Ning Yang, email: yangyn5126@163.com

Yi-Tong Ma, email: myt-xj@163.com

Keywords: long non-coding RNA, acute myocardial infarction, microarray

Received: June 17, 2016     Accepted: February 13, 2017     Published: March 18, 2017

ABSTRACT

In this study, we examined the long noncoding RNA (lncRNA) expression pattern in Uyghur patients (a minority of China) with acute myocardial infarction (AMI) on a genome-wide scale. Total RNAs were extracted from the peripheral blood of 55 Uyghur AMI patients and 55 healthy volunteers. The expression levels of genome-wide scale lncRNAs and mRNAs were determined by microarray in 10 samples (5 AMI and 5 controls). qRT-PCR was used to validate lncRNA expression levels in 100 samples (50 AMI and 50 controls). Data analyses were performed using R and Bioconductor. A total of 3624 up- and 1637 down-regulated lncRNAs were identified to be significantly and differentially expressed between these two groups. The annotation result of their co-expressed mRNAs showed that the most significantly related category of GO analysis was regulation of biological processes, and the most significantly related pathway was apoptosis and its corresponding p53. The microarray identified ENST00000416860.2, ENST00000421157.1 and TCONS_00025701 lncRNAs were confirmed by qRT-PCR. Our study indicated that clusters of lncRNAs were significantly and differentially expressed in the peripheral blood of AMI patients when compared with healthy controls within the Uyghur population. These newly identified lncRNAs may have a potential role in the development of AMI.


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