Oncotarget

Research Papers:

Selenophosphate synthetase 1 (SPS1) is required for the development and selenium homeostasis of central nervous system in chicken (Gallus gallus)

Jin-Long Li, Wei Li, Xue-Tong Sun, Jun Xia, Xue-Nan Li, Jia Lin, Cong Zhang, Xiao-Chen Sun and Shi-Wen Xu _

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Oncotarget. 2017; 8:35919-35932. https://doi.org/10.18632/oncotarget.16283

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Abstract

Jin-Long Li1,2,3,*, Wei Li1,2,3,*, Xue-Tong Sun1,2,3,*, Jun Xia1,2,3, Xue-Nan Li1,2,3, Jia Lin1,2,3, Cong Zhang1,2,3, Xiao-Chen Sun1,2,3, Shi-Wen Xu1,2,3

1College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, P. R. China

2Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, Northeast Agricultural University, Harbin, 150030, P. R. China

3Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, Northeast Agricultural University, Harbin, 150030, P. R. China

*These authors contributed equally to this work

Correspondence to:

Jin-Long Li, email: Jinlongli@neau.edu.cn

Shi-Wen Xu, email: shiwenxu@neau.edu.cn

Keywords: selenophosphate synthetase 1, selenium homeostasis, central nervous system, development, chicken

Received: February 14, 2017     Accepted: March 09, 2017     Published: March 16, 2017

ABSTRACT

Selenophosphate synthetase (SPS) is essential for selenoprotein biosynthesis. In two SPS paralogues, SPS1 was only cloned from a cDNA library prepared from avian organ. However, the biological function of SPS1 in chicken central nervous system (CNS) remains largely unclear. To investigate the role of avian SPS1 in the development and selenium (Se) homeostasis of CNS, fertile eggs, chicken embryos, embryo neurons and chicks were employed in this study. The response of SPS1 transcription to the development and Se levels of CNS tissues was analyzed using qRT-PCR. SPS1 gene exists extensively in the development of chicken CNS. The wide expression of avian SPS1 can be controlled by the Se content levels, which suggests that SPS1 is important in the regulation of Se homeostasis. The fundamental mechanism of these effects is that Se alters the half-life and stability of SPS1 mRNA. Therefore, SPS1 exerts an irreplaceable biological function in chicken CNS and Se homeostasis is closely related to the expression of SPS1. These results suggested that SPS1 was required for the development and Se homeostasis of CNS in chicken.


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