Oncotarget

Research Papers:

Auranofin radiosensitizes tumor cells through targeting thioredoxin reductase and resulting overproduction of reactive oxygen species

Hui Wang, Soumaya Bouzakoura, Sven de Mey, Heng Jiang, Kalun Law, Inès Dufait, Cyril Corbet, Valeri Verovski, Thierry Gevaert, Olivier Feron, Dirk Van den Berge, Guy Storme and Mark De Ridder _

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Oncotarget. 2017; 8:35728-35742. https://doi.org/10.18632/oncotarget.16113

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See the news about this paper: Oncotarget. 2017; 8:35482-3. DOI: 10.18632/oncotarget.16613

Hui Wang1, Soumaya Bouzakoura1, Sven de Mey1, Heng Jiang1, Kalun Law1, Inès Dufait1,2, Cyril Corbet3, Valeri Verovski1, Thierry Gevaert1, Olivier Feron3, Dirk Van den Berge1, Guy Storme1, Mark De Ridder1

1Department of Radiotherapy, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel, Brussels, Belgium

2Laboratory of Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium

3Pole of Pharmacology and Therapeutics (FATH), Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain, Brussels, Belgium

Correspondence to:

Mark De Ridder, email: mark.deridder@uzbrussel.be

Keywords: auranofin, radiosensitization, ROS, thioredoxin reductase, buthionine sulfoximine

Received: September 30, 2016     Accepted: March 01, 2017     Published: March 10, 2017

ABSTRACT

Auranofin (AF) is an anti-arthritic drug considered for combined chemotherapy due to its ability to impair the redox homeostasis in tumor cells. In this study, we asked whether AF may in addition radiosensitize tumor cells by targeting thioredoxin reductase (TrxR), a critical enzyme in the antioxidant defense system operating through the reductive protein thioredoxin. Our principal findings in murine 4T1 and EMT6 tumor cells are that AF at 3–10 μM is a potent radiosensitizer in vitro, and that at least two mechanisms are involved in TrxR-mediated radiosensitization. The first one is linked to an oxidative stress, as scavenging of reactive oxygen species (ROS) by N-acetyl cysteine counteracted radiosensitization. We also observed a decrease in mitochondrial oxygen consumption with spared oxygen acting as a radiosensitizer under hypoxic conditions. Overall, radiosensitization was accompanied by ROS overproduction, mitochondrial dysfunction, DNA damage and apoptosis, a common mechanism underlying both cytotoxic and antitumor effects of AF. In tumor-bearing mice, a simultaneous disruption of the thioredoxin and glutathione systems by the combination of AF and buthionine sulfoximine was shown to significantly improve tumor radioresponse. In conclusion, our findings illuminate TrxR in cancer cells as an exploitable radiobiological target and warrant further validation of AF in combination with radiotherapy.


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