Oncotarget

Research Papers:

High expression of PTBP1 promote invasion of colorectal cancer by alternative splicing of cortactin

Zhi-na Wang, Dan Liu, Bin Yin, Wen-yi Ju, Hui-zhong Qiu, Yi Xiao, Yuan-jia Chen, Chand Khanna and Chong-mei Lu _

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Oncotarget. 2017; 8:36185-36202. https://doi.org/10.18632/oncotarget.15873

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Abstract

Zhi-na Wang1,*, Dan Liu1,2,*, Bin Yin3, Wen-yi Ju3, Hui-zhong Qiu4, Yi Xiao4, Yuan-jia Chen1, Xiao-zhong Peng3 and Chong-mei Lu1

1Department of Gastroenteology and Hepatology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China

2Department of Gastroenterology and Hepatology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China

3National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China

4Department of General Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China

*These authors have contributed equally to this work

Correspondence to:

Chong-mei Lu, email: lcm5788@163.com

Xiao-zhong Peng, email: pengxiaozhong@pumc.edu.cn

Keywords: polypyrimidine tract-binding protein 1, cortactin, colorectal cancer, splicing, invasion

Received: June 13, 2016    Accepted: January 23, 2017    Published: March 03, 2017

ABSTRACT

Polypyrimidine tract-binding protein 1 (PTBP1) involving in almost all steps of mRNA regulation including alternative splicing metabolism during tumorigenesis due to its RNA-binding activity. Initially, we found that high expressed PTBP1 and poor prognosis was interrelated in colorectal cancer (CRC) patients with stages II and III CRC, which widely different in prognosis and treatment, by immunohistochemistry. PTBP1 was also upregulated in colon cancer cell lines. In our study, knockdown of PTBP1 by siRNA transfection decreased cell proliferation and invasion in vitro. Denovirus shRNA knockdown of PTBP1 inhibited colorectal cancer growth in vivo. Furthermore, PTBP1 regulates alternative splicing of many target genes involving in tumorgenesis in colon cancer cells. We confirmed that the splicing of cortactin exon 11 which was only contained in cortactin isoform-a, as a PTBP1 target. Knockdown of PTBP1 decreased the expression of cortactin isoform-a by exclusion of exon 11. Also the mRNA levels of PTBP1 and cortactin isoform-a were cooperatively expressed in colorectal cancer tissues. Knocking down cortactin isoform-a significantly decreased cell migration and invasion in colorectal cancer cells. Overexpression of cortactin isoform-a could rescue PTBP1-knockdown effect of cell motility. In summary the study revealed that PTBP1 facilitates colorectal cancer migration and invasion activities by inclusion of cortactin exon 11.


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