Oncotarget

Research Papers:

A novel bioactive osteogenesis scaffold delivers ascorbic acid, β-glycerophosphate, and dexamethasone in vivo to promote bone regeneration

Chao Wang, Xuecheng Cao and Yongxian Zhang _

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Oncotarget. 2017; 8:31612-31625. https://doi.org/10.18632/oncotarget.15779

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Abstract

Chao Wang1, Xuecheng Cao1 and Yongxian Zhang1

1Department of Orthopedic Injury, General Hospital of Jinan Military Area, Tianqiao District, Jinan, Shangdong, China

Correspondence to:

Yongxian Zhang, email: yongxianzhanggk@sina.com

Keywords: osteogenesis scaffold, ascorbic acid, β-glycerophosphate, dexamethasone, bone regeneration

Received: December 28, 2016     Accepted: January 17, 2017     Published: February 28, 2017

ABSTRACT

Ascorbic acid, β-glycerophosphate, and dexamethasone have been used in osteogenesis differentiation medium for in vitro cell culture, nothing is known for delivering these three bioactive compounds in vivo. In this study, we synthesized a novel bioactive scaffold by combining these three compounds with a lysine diisocyanate-based polyurethane. These bioactive compounds were released from the scaffold during the degradation process. The cell culture showed that the sponge-like structure in the scaffold was critical in providing a large surface area to support cell growth and all degradation products of the polymer were non-toxic. This bioactive scaffold enhanced the bone regeneration as evidenced by increasing the expression of three bone-related genes including collagen type I, Runx-2 and osteocalcin in rabbit bone marrow stem cells (BMSCs) in vitro and in vivo. The osteogenesis differentiation of BMSCs cultured in this bioactive scaffold was similar to that in osteogenesis differentiation medium and more extensive in this bioactive scaffold compared to the scaffold without these three bioactive compounds. These results indicated that the scaffold containing three bioactive compounds was a good osteogenesis differentiation promoter to enhance the osteogenesis differentiation and new bone formation in vivo.


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PII: 15779