Oncotarget

Research Papers:

Exploring the role of Paraoxonase-2 in bladder cancer: analyses performed on tissue samples, urines and cell culturess

Tiziana Bacchetti, Davide Sartini, Valentina Pozzi, Tiziana Cacciamani, Gianna Ferretti _ and Monica Emanuelli

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Oncotarget. 2017; 8:28785-28795. https://doi.org/10.18632/oncotarget.15674

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Abstract

Tiziana Bacchetti1,*, Davide Sartini2,*, Valentina Pozzi3, Tiziana Cacciamani1, Gianna Ferretti2, Monica Emanuelli2,3

1Department of Life and Environmental Sciences, Polytechnic University of Marche, Ancona, Italy

2Department of Clinical Sciences Polytechnic University of Marche, Ancona, Italy

3New York-Marche Structural Biology Center (NY-MaSBiC), Polytechnic University of Marche, Ancona, Italy

*These authors have contributed equally to this work

Correspondence to:

Gianna Ferretti, email: g.ferretti@univpm.it

Keywords: paraoxonase-2, oxidative stress, bladder cancer, biomarker, antioxidant

Received: October 24, 2016    Accepted: February 06, 2017    Published: February 24, 2017

ABSTRACT

The enzyme paraoxonase-2 (PON2) is ubiquitously expressed and exerts its antiapoptotic and antioxidative functions in several intracellular compartments.

The aim of this study is to investigate the role of PON2 in bladder cancer (BC). The expression levels of PON2 in paired tumor and normal bladder tissue samples and in urinary exfoliated cells from patients affected with BC and healthy donors were evaluated. Moreover, the effect of PON2 overexpression on tumor cell proliferation and susceptibility to oxidative stress was investigated in human bladder cancer cell line T24.

Our results showed that PON2 expression levels were significantly higher in BC compared with non-tumor tissue. In urinary exfoliated cells from BC patients, PON2 mRNA levels showed an inverse correlation with tumor stage (pT). Moreover, PON2 overexpression in T24 cells led to a significant increase in tumor cell proliferation and resistance to oxidative stress.

The results obtained showed that PON2 could represent a molecular biomarker for bladder cancer and suggest a potential role of the enzyme as a prognostic factor for this neoplasm.


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