Adipophilin as prognostic biomarker in clear cell renal cell carcinoma
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Yuri Tolkach1,*, Christine Lüders1,*, Sebastian Meller1, Klaus Jung2,3, Carsten Stephan2,3, Glen Kristiansen1
1Institute for Pathology, University Hospital Bonn, Bonn, Germany
2Berlin Institute of Urologic Research, Berlin, Germany
3Department of Urology, Charité, Universitätsmedizin Berlin, Berlin, Germany
*These authors have contributed equally to this work
Glen Kristiansen, email: firstname.lastname@example.org
Keywords: renal cell carcinoma, clear cell, adipophilin, perilipin 2, PLIN2
Received: December 12, 2016 Accepted: January 27, 2017 Published: February 23, 2017
Objective: To study the expression of adipophilin (PLIN2), a lipid storage-associated cell protein, in different subtypes of renal cell cancer and to elucidate its prognostic value.
Materials and Methods: Two-hundred-seventy-five patients with renal cell carcinoma (RCC) were included in this study. Immunohistochemistry with a polyclonal antibody to adipophilin was used on the tissue microarray (formalin-fixed, paraffin-embedded tissue) for detection of adipophilin. Median follow-up time was 91 (range 1-159) months in the whole cohort and 100 (1-159) months for patients with clear-cell RCC. Additional validation for adipophilin was performed using publicly available gene expression data for clear cell RCC from The Cancer Genome Atlas (TCGA).
Results: Adipophilin expression was detected in 14.3% of papillary RCC, in 0% of chromophobe RCC and in 58.7% of clear-cell RCC in the cytoplasm or at the membrane. Only membrane expression was correlated with other clinical parameters (pT-stage, pN-stage, R-status, sex) and showed a prognostic significance in univariate analysis with regard to overall survival of patients with clear cell subtype (HR 2.90, 95% CI 1.55-5.42, p=0.001), which failed significance on multivariate analysis. mRNA expression of PLIN2 on TCGA data using best selected cut-off was prognostically significant in both univariate (HR 1.76, 95% CI 1.28-2.42, p = 0.0005) and multivariate analyses (HR 1.46, 95% CI 1.05-2.04, p = 0.0257).
Conclusions: Adipophilin is a novel and still understudied prognostic biomarker in clear cell renal cell carcinoma which deserves further study.
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