Granulocyte colony-stimulating factor enhances the therapeutic efficacy of bone marrow mesenchymal stem cell transplantation in rats with experimental acute pancreatitis
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Bo Qu1, Yanjie Chu1, Fang Zhu2, Beibei Wang3, Ting Liu1, Bo Yu4, Shizhu Jin1
1Department of Gastroenterology and Hepatology, The Second Affiliated Hospital, Harbin Medical University, Harbin, 150086, China
2Department of Gastroenterology and Hepatology, The First People's Hospital of Yongkang, Zhejiang Province, 321300, China
3Department of the Second Internal Medicine Ward, The Yellow River Hospital, Tianjin, 300101, China
4Department of Gastroenterology and Hepatology, The Tailai County People's Hospital, Heilongjiang Province, 162400, China
Shizhu Jin, email: [email protected]
Keywords: bone marrow stem cell transplant, granulocyte colony-stimulating factor, acute pancreatitis, transdifferentiation
Received: November 24, 2016 Accepted: February 07, 2017 Published: February 19, 2017
Introduction: Acute pancreatitis (AP) is one of the most common diseases involving necrotic inflammation. Bone marrow mesenchymal stem cells (BMMSCs) have the potential of multi-directional differentiation and self-renewal for tissue repair. It remains less clear if granulocyte colony-stimulating factor (G-CSF) can improve the therapeutic effect of BMMSC transplant in AP. Therefore, we explored this issue in a rat model of experimental AP.
Results: Transplanted PKH26-positive BMMSCs were present in the injured pancreatic tissue, with some cells co-expressed pancreatic cellular markers, including Pax-4, Ngn3 and Nkx-6. Pathological, biochemical and serological data suggested an improvement in histological and functional recovery in these animals relative to control. Overall, the AP model rats received BMMSCs and G-CSF co-treatment showed better recovery in terms of tissue regeneration and blood biochemical levels relative to other groups.
Materials and Methods: BMMSCs from donor rats were labeled with the fluorescent dye PKH26 and transfused into recipient rats with AP induced by L-arginine. The animals were divided into a control group, and groups treated with BMMSCs, G-CSF, and BMMSCs together with G-CSF. Therapeutic effects were evaluated histologically with immunohistochemistry and immunofluorescence, together with biochemical measurement of pancreatic markers.
Conclusion: G-CSF therapy with BMMSC transplantation improves histological and functional outcomes in rats with experimental AP.
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