Oncotarget

Research Papers: Pathology:

The effects of high glucose on tendon-derived stem cells: implications of the pathogenesis of diabetic tendon disorders

Yu-Cheng Lin, Ying-Juan Li, Yun-Feng Rui _, Guang-Chun Dai, Liu Shi, Hong-Liang Xu, Ming Ni, Song Zhao, Hui Chen, Chen Wang, Gang Li and Gao-Jun Teng

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Oncotarget. 2017; 8:17518-17528. https://doi.org/10.18632/oncotarget.15418

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Abstract

Yu-Cheng Lin1,*, Ying-Juan Li2,*, Yun-Feng Rui1,3,4, Guang-Chun Dai1, Liu Shi1,5,6, Hong-Liang Xu3, Ming Ni7, Song Zhao1, Hui Chen1, Chen Wang1, Gang Li5,6 and Gao-Jun Teng8

1 Department of Orthopaedics, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, Jiangsu, China

2 Department of Geriatrics, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, China

3 Department of Orthopaedics, Xishan People’s Hospital, Wuxi, Jiangsu, China

4 China Orthopedic Regenerative Medicine Group, China

5 Department of Orthopaedics and Traumatology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, SAR, China

6 Program of Stem Cell and Regeneration, School of Biomedical Science, and Li Ka Shing Institute of Health Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, SAR, China

7 Department of Orthopaedics, The General Hospital of Chinese People’s Liberation Army, Beijing, China

8 Department of Radiology, Jiangsu Key Laboratory of Molecular and Functional Imaging, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, Jiangsu, China

* These authors have contributted equally to this work

Correspondence to:

Yun-Feng Rui, email:

Gao-Jun Teng, email:

Keywords: tendon-derived stem cells; high glucose; diabetic tendon disorders; pathogenesis; Pathology Section

Received: November 30, 2016 Accepted: February 07, 2017 Published: February 16, 2017

Abstract

Patients with diabetes are at great risk to suffer many musculoskeletal disorders, such as tendinopathy, tendon rupture and impaired tendon healing. However, the pathogenesis of these tendon disorders still remains unclear. In this study, we aimed to investigate the effects of high glucose on cell proliferation, cell apoptosis and tendon-related markers expression of tendon-derived stem cells (TDSCs) in vitro. These findings might provide new insights into the pathogenesis of diabetic tendon disorders. The cell proliferative ability and apoptosis rate of TDSCs in different groups were evaluated by MTT assay and Annexin V-FITC/PI staining assay. The mRNA expression of tendon-related markers (Scleraxis and Collagen I alpha 1 chain) were assessed by qRT-PCR. The protein expression of tendon-related markers (Tenomodulin and Collagen I) were measured by Western blotting. The proliferative ability of TDSCs treated with high glucose (15mM and 25mM) decreased significantly at day1, day3 and day5. The cell apoptosis of TDSCs increased significantly when they were cultured with high glucose for 48h in vitro. The gene expression of Scleraxis and Collagen I alpha 1 chain in TDSCs decreased significantly when they were treated with high glucose for 24h and 48h. The protein expression of Tenomodulin and Collagen I in TDSCs decreased significantly when they were treated with high glucose for 24h and 48h. High glucose could inhibit cell proliferation, induce cell apoptosis and suppress the tendon-related markers expression of TDSCs in vitro. These findings might account for some pathological mechanisms underlying the pathogenesis of diabetic tendon disorders.


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