Oncotarget

Research Papers:

Circulating microRNA-422a is associated with lymphatic metastasis in lung cancer

Li-Na Wu, Bo Hu, Bing-Tian Zhao, Yi-Nan Liu, Yue Yang, Li-Jian Zhang and Jin-Feng Chen _

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Oncotarget. 2017; 8:42173-42188. https://doi.org/10.18632/oncotarget.15025

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Abstract

Lina Wu1,*, Bo Hu2,*, Bingtian Zhao3,*, Yinan Liu2, Yue Yang2, Lijian Zhang2 and Jinfeng Chen2

1Central Laboratory, Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education, Beijing Cancer Hospital & Institute, Peking University Cancer Hospital, Beijing, China

2Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education, Department of Thoracic Surgery II, Peking University School of Oncology, Beijing Cancer Hospital & Institute, Beijing, China

3Department of Respiratory & Critical Care Medicine, Tianjin Chest Hospital, Tianjin, China

*These authors have contributed equally to this work

Correspondence to:

Jinfeng Chen, email: chenjinfengdoctor@163.com

Keywords: microRNA, miR-422a, lung cancer, lymphatic metastasis, biomarker

Received: August 15, 2016     Accepted: January 10, 2017     Published: February 02, 2017

ABSTRACT

To identify specific circulating microRNAs that were associated with the lymphatic metastasis in lung cancer, we performed miRNA microarray analysis of lymph node with and without metastasis from five lung cancer patients. Top six differentially expressed miRNAs were selected for further validation. A training cohort of 26 patients with lung cancer was firstly recruited and the selected miRNAs in the plasma samples were investigated. miRNA-422a, with highest diagnostic accuracy in lymphatic metastasis was identified (AUC, area under the receiver operating characteristic curve, 0.744; 95%CI, 0.570-0.918). The diagnostic value of miR-422a was also demonstrated by a validation cohort of 51 lung cancer patients (AUC, 0.880; 95%CI, 0.787-0.972). Moreover, a high diagnostic value was also observed after integrated analysis of training and validation cohorts (AUC, 0.792; 95%CI, 0.688-0.896). The odds ratio of high miR-422a expression for lymphatic metastasis in lung cancer was 13.645 (95%CI, 2.677-69.553) after adjustment of the potential confounding factors. Furthermore, we predicted the target genes of miR-422a by combining the online database, miRcords, and the data from GEO and TCGA. Sixty-one target genes of miR-422a that might be involved in lymphatic metastasis in lung cancer were identified. And GO analysis suggested multiple target genes relatively concentrated in the biological processes of apoptosis, transport, and protein phosphorylation.


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