Oncotarget

Research Papers:

Subdiaphragmatic vagotomy promotes tumor growth and reduces survival via TNFα in a murine pancreatic cancer model

Lars Ivo Partecke, André Käding _, Dung Nguyen Trung, Stephan Diedrich, Matthias Sendler, Frank Weiss, Jens-Peter Kühn, Julia Mayerle, Katharina Beyer, Wolfram von Bernstorff, Claus-Dieter Heidecke and Wolfram Keßler

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Oncotarget. 2017; 8:22501-22512. https://doi.org/10.18632/oncotarget.15019

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Abstract

Lars Ivo Partecke1,*, André Käding1,*, Dung Nguyen Trung1, Stephan Diedrich1, Matthias Sendler2, Frank Weiss2, Jens-Peter Kühn3, Julia Mayerle2, Katharina Beyer4, Wolfram von Bernstorff1, Claus-Dieter Heidecke1, Wolfram Keßler1

1Department of General, Visceral, Thoracic and Vascular Surgery, University Medicine, Greifswald, Germany

2Department of Internal Medicine A, University Medicine, Greifswald, Germany

3Department of Experimental Radiology, University Medicine, Greifswald, Germany

4Current address: Department of General, Visceral and Vascular Surgery, Charité–University Medicine, Campus Benjamin Franklin, Berlin, Germany

*These authors contributed equally to this work and share first authorship

Correspondence to:

André Käding, email: andre.kaeding@uni-greifswald.de

Keywords: vagus nerve, TNFα, pancreatic cancer, macrophages, murine cancer model

Received: September 06, 2016     Accepted: January 23, 2017     Published: February 02, 2017

ABSTRACT

This study analyses the effects of vagotomy on tumor growth and survival in a murine, pancreatic cancer model in wild-type and TNFα-knockout (–/–) mice.

Throughout many operative procedures in the upper gastrointestinal tract the partial or complete transection of the vagus nerve or its local nerve fibers is unavoidable. Thereby its anti-inflammatory effects in residual tumor tissue may get lost. This effect may be mediated by tumor-associated macrophages (TAM) secreting TNFα.

In an orthotopic murine pancreatic cancer model subdiaphragmatic vagotomy versus sham surgery was performed. The impact on tumor growth was monitored in wild type and TNFα –/– mice using MRI. TAMs as well as expression levels of TNFα were analyzed using immunohistochemistry. The role of TNFα on tumor growth and migration was examined in vitro. Vagotomised mice showed increased tumor growth with macroscopic features of invasive growth and had a shorter survival time. The loss of vagal modulation led to significantly increased TNFα levels in tumors and considerably elevated numbers of TAMs. In vitro TNFα significantly stimulated growth (p < 0.05) and migration (p < 0.05) of pancreatic cancer cells. TNFα –/– mice survived significantly longer after tumor implantation (p < 0.05), with vagotomy not affecting the prognosis of these animals (p > 0.05).

Vagotomy can increase tumor growth and worsen survival in a murine pancreatic cancer model mediated through TAMs and TNFα. Hence, the suppression of TAMs and the modulation of TNFα dependent pathways could offer new perspectives in immunotherapies of pancreatic cancer patients especially with remaining vital tumor cells and lost vagal modulation.


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