Comparative analysis of microRNA expression profiles between A549, A549/DDP and their respective exosomes
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Xiaobing Qin1,2,*, Shaorong Yu1,*, Xiaoyue Xu1, Bo Shen1 and Jifeng Feng1
1Research Center for Clinical Oncology, Nanjing Medical University Affiliated Cancer Hospital, Jiangsu Cancer Hospital and Jiangsu Institute of Cancer Research, Nanjing, Jiangsu Province, China
2Department of Oncology, Xuzhou First People’s Hospital, Xuzhou, Jiangsu Province, China
*These authors have contributed equally to this work
Keywords: lung cancer, microRNA, exosomes, drug resistance, cisplatin
Received: September 08, 2016 Accepted: January 09, 2017 Published: February 02, 2017
Exosomes were reported to transport bioactive molecules and influence the biology behavior of recipient cells. In order to study the role of exosomal microRNAs in the mechanism of cisplatin resistance to lung cancer cells, we analyzed the expression profiles of microRNAs in A549, A549/DDP cells and their exosomes by microarray. The results showed that a certain proportion of microRNAs were co-expressed in the cells and exosomes. Linear regression analysis showed that the expression of microRNAs in A549 and A549/DDP cells were strongly correlated with those in their respective exosomes. The expression level of 5 microRNAs (miR-197-5p, miR-4443, miR-642a-3p, miR-27b-3p and miR-100-5p) with the most differential expression were verified by qRT-PCR. The results were consistent with those of the microarray. Target gene prediction and pathway analysis discovered that the microRNAs in the intersections may participate in drug resistance. And the prediction of their association with diseases found that most of these microRNAs was associated with lung cancer. We could draw a preliminary conclusion that microRNAs in exosomes may be involved in the drug resistance of lung cancer cells to cisplatin.
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