Exosome secretome and mediated signaling in breast cancer patients with nontuberculous mycobacterial disease
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Julie V. Philley1, Anbarasu Kannan2, David E. Griffith1, Megan S. Devine1, Jeana L. Benwill1, Richard J. Wallace Jr1,3, Barbara A. Brown-Elliott3, Foram Thakkar1, Varsha Taskar1, James G. Fox1, Ammar Alqaid1, Hernaina Bains1, Sudeep Gupta4, Santanu Dasgupta2
1Departments of Medicine, The University of Texas Health Science Center at Tyler, Texas, USA
2Cellular and Molecular Biology, The University of Texas Health Science Center at Tyler, Texas, USA
3The Mycobacteria/Nocardia Research Laboratory Department of Microbiology, The University of Texas Health Science Center at Tyler, Texas, USA
4Medical Oncology, Tata Memorial Center, Mumbai, India
Santanu Dasgupta, email: firstname.lastname@example.org
Keywords: Exosomes, breast cancer, nontuberculous Mycobacterium, ECM1, biomarker
Received: July 10, 2016 Accepted: January 10, 2017 Published: February 01, 2017
Bronchiectasis Nontuberculous mycobacterium (NTMnb) infection is an emerging health problem in breast cancer (BCa) patients. We measured sera exosome proteome in BCa-NTMnb subjects and controls by Mass Spectroscopy. Extracellular matrix protein 1 (ECM1) was detected exclusively in the circulating exosomes of 82% of the BCa-NTMnb cases. Co-culture of ECM1+ exosomes with normal human mammary epithelial cells induced epithelial to mesenchymal transition accompanied by increased Vimentin/CDH1 expression ratio and Glutamate production. Co-culture of the ECM1+ exosomes with normal human T cells modulated their cytokine production. The ECM1+ exosomes were markedly higher in sera obtained from BCa-NTMnb subjects. Exclusive expression of APN, APOC4 and AZGP1 was evident in the circulating exosomes of these BCa-NTMnb cases, which predicts disease prevalence independent of the body max index in concert with ECM1. Monitoring ECM1, APN, APOC4 and AZGP1 in the circulating exosomes could be beneficial for risk assessment, monitoring and surveillance of BCa-NTMnb.
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