Research Papers:
Posttranscriptional regulation of Galectin3 by miR128 contributes to colorectal cancer progression
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Weiqun Lu1,*, Jia Wang1,*, Guohua Yang1, Nanrong Yu1, Zhiliang Huang1, Houwei Xu1, Jianchang Li1, Jiliang Qiu1, Xiang Zeng1, Shicai Chen1, Nan Li1, Haiying Liu1
1Department of Gastrointestinal Neoplasms Surgery, Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou 510095, Guangdong, China
*These authors contributed equally to this work
Correspondence to:
Nan Li, email: [email protected]
Haiying Liu, email: [email protected]
Keywords: colorectal cancer, Galectin-3, miR-128, cancer progression
Received: December 06, 2016 Accepted: January 17, 2017 Published: January 27, 2017
ABSTRACT
Here we demonstrated that Galectin-3 protein level was frequently up-regulated in colorectal cancer (CRC) cells and tissues. Galectin-3 up-regulation correlated with CRC progression and predicted a shorter overall survival of CRC patients. Galectin-3 overexpression attenuated the chemo-sensitivity of cancer cells, but enhanced the potential invasiveness. To explore the mechanism for Galectin-3 dysregulation, we found that miR-128 level was frequently down-regulated in CRC and negatively correlated with Galectin-3 level. Using bioinformatics analysis and experimental validation, we showed that miR-128 could directly target Galectin-3 to repress its protein level. MiR-128 decrease associated with CRC progression and predicted a worse overall survival of CRC patients. Ectopic miR-128 expression enhanced the chemo-sensitivity of CRC cells in vitro and in vivo, and inhibited the potential invasiveness. Galectin-3 expression impaired the cancer suppressive effects of miR-128. These data highlighted the role of miR-128/Galectin-3 axis in colorectal cancer.