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Overexpression of lncRNA ANRIL up-regulates VEGF expression and promotes angiogenesis of diabetes mellitus combined with cerebral infarction by activating NF-κB signaling pathway in a rat model

Bo Zhang, Dan Wang, Tie-Feng Ji, Lei Shi and Jin-Lu Yu _

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Oncotarget. 2017; 8:17347-17359. https://doi.org/10.18632/oncotarget.14468

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Abstract

Bo Zhang1,*, Dan Wang2,*, Tie-Feng Ji3, Lei Shi1 and Jin-Lu Yu1

1 Department of Neurosurgery, The First Hospital of Jilin University, Changchun, P.R. China

2 Department of Ophthalmology, The First Hospital of Jilin University, Changchun, P.R. China

3 Department of Radiology, The First Hospital of Jilin University, Changchun, P.R. China

* These authors have contributed equally to the manuscript

Correspondence to:

Jin-Lu Yu, email:

Keywords: LncRNA ANRIL; diabetes mellitus; cerebral infarction; NF-κB signaling pathway; vascular endothelial growth factor

Received: July 27, 2016 Accepted: December 07, 2016 Published: January 03, 2017

Abstract

Objective: This study aimed to explore the effects of lncRNA ANRIL on vascular endothelial growth factor (VEGF) and angiogenesis in diabetes mellitus (DM) combined with cerebral infarction (CI) through NF-κB signaling pathway.

Methods: Adult male Wistar rats were randomly divided into control group and DM + CI group, and the DM + CI group were subdivided into Vector, shANRIL, PDTC, pcDNA-ANRIL, and pcDNA-ANRIL + PDTC groups. VEGF and FMS-like tyrosine kinase (FLT-1) expressions were measured by immunohistochemistry and endothelium dependent microvessel density (MVD) was detected by differentiation 31 (CD31) and para-amiuosalicylic acid (PAS) double staining. The qRT-PCR was applied to measure mRNA expressions of VEGF, FLT-1, Kinase insert domain protein receptor (FLK-1) and NF-κB, and Western blotting was conducted to detected expressions of VEGF, NF-κB and p-IκB/IκB.

Results: Compared with the control group, protein expressions of VEGF, NF-κB, p-IκB/IκB, expression of ANRIL, and mRNA expressions of VEGF, FLT-1 and NF-κB were increased in the DM + CI group. Compared with the Vector group, protein expressions of VEGF, NF-κB, p-IκB/IκB, expression of ANRIL, mRNA expressions of VEGF, FLT-1 and NF-κB, and endothelium dependent MVD were increased in the pcDNA-ANRIL group, while decreased in the shANRIL group and PDTC group. Compared with the pcDNA-ANRIL group, protein expressions of VEGF, NF-κB, p-IκB/IκB, expression of ANRIL, mRNA expressions of VEGF, FLT-1 and NF-κB, and endothelium dependent MVD were decreased in the pcDNA-ANRIL + PDTC group.

Conclusion: Overexpressed lncRNA ANRIL upregulates VEGF and promotes angiogenesis by activating NF-κB signaling pathway in DM + CI rats. .


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