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Protective effect of tanshinone IIA against cardiac hypertrophy in spontaneously hypertensive rats through inhibiting the Cys-C/Wnt signaling pathway

Jun Feng _, Hua-Wen Chen, Li-Juan Pi, Jin Wang and Da-Qian Zhan

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Oncotarget. 2017; 8:10161-10170. https://doi.org/10.18632/oncotarget.14328

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Abstract

Jun Feng1, Hua-Wen Chen1, Li-Juan Pi1, Jin Wang1, Da-Qian Zhan1

1Department of Emergency Medicine, Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030, Hubei, P.R. China

Correspondence to:

Hua-Wen Chen, email: ellewzh443@163.com

Keywords: tanshinone IIA, cardiac hypertrophy, Cys-C/Wnt signaling pathway, spontaneously hypertensive rats

Received: July 30, 2016     Accepted: November 09, 2016     Published: December 28, 2016

ABSTRACT

The study aimed to investigate the protective effect of tanshinone IIA against cardiac hypertrophy in spontaneously hypertensive rats (SHRs) through the Cys-C/Wnt signaling pathway. Thirty SHRs were randomly divided into cardiac hypertrophy, low- and high-dose tanshinone IIA groups. Ten Wistar-Kyoto rats were selected as control group. The systolic blood pressure (SBP), heart weight (HW), left ventricular weight (LVW) and body weight (BW) of all rats were recorded. HE staining and qRT-PCR were applied to observe the morphology of myocardial tissue and mRNA expressions of COL1A1 and COL3A1. ELISA and Western blotting were used to measure the serum asymmetric dimethylarginine (ADMA), nitric oxide (NO) and cardiac troponin I (cTnI) levels, and the expressions of the Cys-C/Wnt signaling pathway-related proteins, eNOS and Nox4. Compared with the cardiac hypertrophy group, the SBP, HW/BW, LVW/BW, swelling degree of myocardial cells, COL1A1 and COL3A1 mRNA expressions, serum cTnI and ADMA levels, and the Cys-C/Wnt signaling pathway-related proteins and Nox4 expressions in the low- and high-dose tanshinone IIA groups were decreased, but the endothelial NO synthase (eNOS), phosphorylated eNOS (Ser1177) and NO expressions were increased. No significant difference was found between the low- and high-dose tanshinone IIA groups. Our study indicated a protective effect of tanshinone IIA against cardiac hypertrophy in SHRs through inhibiting the Cys-C/Wnt signaling pathway.


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