A six-microRNA panel in plasma was identified as a potential biomarker for lung adenocarcinoma diagnosis
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Xin Zhou1,*, Wei Wen2,*, Xia Shan3,*, Wei Zhu1, Jing Xu1, Renhua Guo1, Wenfang Cheng4, Fang Wang5, Lian-Wen Qi6, Yan Chen7, Zebo Huang1, Tongshan Wang1, Danxia Zhu8, Ping Liu1,9, Yongqian Shu1,9
1Department of Oncology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, PR China
2Department of Thoracic Surgery, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, PR China
3Department of Respiration, The Affiliated Jiangning Hospital of Nanjing Medical University, Nanjing 210000, PR China
4Department of Gastroenterology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, PR China
5Department of Cardiology, First Affiliated Hospital of Nanjing Medical University,Nanjing 210029, PR China
6State Key Laboratory of Natural Medicines and Department of Pharmacognosy, China Pharmaceutical University, Nanjing, 210009, China
7Department of Emergency, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, PR China
8Department of Oncology, The Third Affiliated Hospital of Soochow University, Changzhou 213003, China
9Cancer Center of Nanjing Medical University, Nanjing 210029, China
*These authors contributed equally to this work
Wei Zhu, email: email@example.com
Ping Liu, email: firstname.lastname@example.org
Yongqian Shu, email: email@example.com
Keywords: plasma, miRNA, lung adenocarcinoma, diagnosis, exosomes
Received: July 25, 2016 Accepted: December 13, 2016 Published: December 27, 2016
Differently expressed microRNAs (miRNAs) in the plasma of lung adenocarcinoma (LA) patients might serve as biomarkers for LA detection. MiRNA expression profiling was performed using Exiqon panels followed by the verification (30 LA VS. 10 healthy controls (HCs)) with quantitative reverse transcription polymerase chain reaction (qRT-PCR) in the screening phase. Identified miRNAs were confirmed through training (42 LA VS. 32 HCs) and testing stages (66 LA VS. 62 HCs) by using qRT-PCR based absolute quantification methods. A total of six up-regulated plasma miRNAs (miR-19b-3p, miR-21-5p, miR-221-3p, miR-409-3p, miR-425-5p and miR-584-5p) were identified. The six-miRNA panel could discriminate LA patients from HCs with areas under the receiver operating characteristic curve of 0.72, 0.74 and 0.84 for the training, testing and the external validation stage (33 LA VS. 30 HCs), respectively. All the miRNAs identified except miR-584-5p were significantly up-regulated in LA tissues. MiR-19-3p, miR-21-5p, miR-409-3p and miR-425-5p showed high expression in arterial plasma with borderline significance. Additionally, miR-19-3p, miR-21-5p and miR-221-3p were significantly up-regulated in exosomes extracted from LA peripheral plasma samples. In conclusion, we identified a six-miRNA panel in peripheral plasma which might give assistance to the detection of LA at least for Asian population to a certain extent.
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