Heterogeneity in sarcoma cell lines reveals enhanced motility of tetraploid versus diploid cells
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Mohamed Jemaà1,2, Samer Abdallah1,2, Gwendaline Lledo1,2, Gaelle Perrot3, Tom Lesluyes3, Catherine Teyssier4, Pierre Roux1,2, Juliette van Dijk1,2, Frederic Chibon3,5, Ariane Abrieu1,2, Nathalie Morin1,2
1Universités de Montpellier, 34293 Montpellier, France
2CRBM, CNRS, UMR 5237, 34293 Montpellier, France
3INSERM U1218, Bergonié Cancer Institute, F-33076 Bordeaux, France
4U1194 INSERM, IRCM, 34298 Montpellier, France
5Department of Pathology, Bergonié Cancer Institute, F-33076 Bordeaux, France
Abrieu Ariane, email: firstname.lastname@example.org
Morin Nathalie, email: email@example.com
Keywords: sarcoma, CINSARC, mitosis, motility, diploid/tetraploid
Received: May 13, 2016 Accepted: November 30, 2016 Published: December 27, 2016
Soft tissue sarcomas with complex genomics are very heterogeneous tumors lacking simple prognosis markers or targeted therapies. Overexpression of a subset of mitotic genes from a signature called CINSARC is of bad prognosis, but the significance of this signature remains elusive. Here we precisely measure the cell cycle and mitosis duration of sarcoma cell lines and we found that the mitotic gene products overexpression does not reflect variation in the time spent during mitosis or G2/M. We also found that the CINSARC cell lines, we studied, are composed of a mixture of aneuploid, diploid, and tetraploid cells that are highly motile in vitro. After sorting diploid and tetraploid cells, we showed that the tetraploid cell clones do not possess a proliferative advantage, but are strikingly more motile and invasive than their diploid counterparts. This is correlated with higher levels of mitotic proteins overexpression. Owing that mitotic proteins are almost systematically degraded at the end of mitosis, we propose that it is the abnormal activity of the mitotic proteins during interphase that boosts the sarcoma cells migratory properties by affecting their cytoskeleton. To test this hypothesis, we designed a screen for mitotic or cytoskeleton protein inhibitors affecting the sarcoma cell migration potential independently of cytotoxic activities. We found that inhibition of several mitotic kinases drastically impairs the CINSARC cell invasive and migratory properties. This finding could provide a handle by which to selectively inhibit the most invasive cells.
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