Long non-coding RNA AK058003, as a precursor of miR-15a, interacts with HuR to inhibit the expression of γ-synuclein in hepatocellular carcinoma cells
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Xiaoqin He1,*, Yongfa Zheng1,*, Yuefeng Zhang2, Yuanyuan Gan1, Yujie Zhou1, Huilin Liang1, Dongcheng Wu3, Wei Ge1, Junjian Deng1, Ximing Xu1
1Cancer Center, Renmin Hospital of Wuhan University, Wuhan, China
2Department of Surgery, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China
3Department of Biochemistry and Molecular Chemistry, School of Basic Medicial Sciences, Wuhan University, Wuhan, China
*These authors have contributed equally to this work
Ximing Xu, email: [email protected]
Keywords: liver cancer, tumor suppressor, lncRNA, mRNA stabilizing protein
Received: October 07, 2016 Accepted: December 15, 2016 Published: December 27, 2016
Long non-coding RNAs (lncRNAs) have been identified as critical players in multiple cancers and lncRNAs are tightly linked to cancer progression. However, only little amount of lncRNAs have been identified to participate in the molecular mechanisms of the progression of hepatocellular carcinoma. In this study, we found that lncRNA-AK058003 is down-regulated in hepatocellular carcinoma tissues and it is associated with the relapse and metastasis of the cancer. Furthermore, lncRNA-AK058003 acts as a tumor suppressor, suppressing hepatocellular carcinoma cell proliferation and metastasis in vitro and in vivo. lncRNA-AK058003 can reduce mRNA stabilizing protein HuR, which results in the inhibition of the expression of γ-synuclein. In addition, a bioinformatics study indicated that γ-synuclein is a target of miR-15a. To verify whether lncRNA-AK058003 plays a role in miR-15a-mediated inhibition of γ-synuclein, we demonstrated that lncRNA-AK058003 is very likely to be a precursor of miR-15a. Collectively, lncRNA-AK058003 can reduce the expression of mRNA stabilizing protein HuR and act as a precursor of miR-15a to suppress γ-synuclein-mediated cell proliferation and the metastasis of hepatocellular carcinoma.
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