Oncotarget

Research Papers:

Mutations acquired by hepatocellular carcinoma recurrence give rise to an aggressive phenotype

Ji-Hye Choi _, Min Jae Kim, Yong Keun Park, Jong-Yeop Im, So Mee Kwon, Hyung Chul Kim, Hyun Goo Woo and Hee-Jung Wang

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Oncotarget. 2017; 8:22903-22916. https://doi.org/10.18632/oncotarget.14248

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Abstract

Ji-Hye Choi1,2,*, Min Jae Kim1,2,*, Yong Keun Park4, Jong-Yeop Im1,2, So Mee Kwon1,2, Hyung Chul Kim3, Hyun Goo Woo1,2, Hee-Jung Wang5

1Department of Physiology, Ajou University School of Medicine, Suwon, Korea

2Department of Biomedical Science, Graduate School, Ajou University, Suwon, Korea

3Developmental Therapeutics Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States

4Department of Surgery, Catholic Kwandong University International St. Mary's Hospital, Incheon, Korea

5Department of Surgery, Ajou University School of Medicine, Suwon, Korea

*These authors have contributed equally to this work

Correspondence to:

Hyun Goo Woo, email: hg@ajou.ac.kr

Hee-Jung Wang, email: wanghj@ajou.ac.kr

Keywords: RNA-Seq, GOLGB1, SF3B3, recurrence, mutation

Received: September 09, 2016     Accepted: December 01, 2016     Published: December 27, 2016

ABSTRACT

Recurrence of hepatocellular carcinoma (HCC) even after curative resection causes dismal outcomes of patients. Here, to delineate the driver events of genomic and transcription alteration during HCC recurrence, we performed RNA-Seq profiling of the paired primary and recurrent tumors from two patients with intrahepatic HCC. By comparing the mutational and transcriptomic profiles, we identified somatic mutations acquired by HCC recurrence including novel mutants of GOLGB1 (E2721V) and SF3B3 (H804Y). By performing experimental evaluation using siRNA-mediated knockdown and overexpression constructs, we demonstrated that the mutants of GOLGB1 and SF3B3 can promote cell proliferation, colony formation, migration, and invasion of liver cancer cells. Transcriptome analysis also revealed that the recurrent HCCs reprogram their transcriptomes to acquire aggressive phenotypes. Network analysis revealed CXCL8 (IL-8) and SOX4 as common downstream targets of the mutants. In conclusion, we suggest that the mutations of GOLGB1 and SF3B3 are potential key drivers for the acquisition of an aggressive phenotype in recurrent HCC.


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