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Research Papers:

Lycium barbarum polysaccharides inhibit proliferation and migration of bladder cancer cell lines BIU87 by suppressing Pi3K/AKT pathway

Xian-Jun Zhang, Hong-Yuan Yu, Yong-jian Cai and Mang Ke _

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Oncotarget. 2017; 8:5936-5942. https://doi.org/10.18632/oncotarget.13963

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Abstract

Xian-Jun Zhang1, Hong-Yuan Yu1, Yong-jian Cai1, Mang Ke1

1Department of Urology, Taizhou Hospital of Zhejiang Province, Wenzhou Medical University, Linhai, Zhejiang Province, PR China

Correspondence to:

Mang Ke, email: kemengtaizhou@163.com

Keywords: lycium barbarum polysaccharides, bladder cancer, Pi3K/AKT, proliferation, migration

Received: November 15, 2016     Accepted: December 09, 2016     Published: December 15, 2016

ABSTRACT

The aim of this study was to verify whether Lycium barbarum polysaccharides inhibits proliferation and migration of BIU87 cells through Pi3K/AKT pathway. Different concentrations of Lycium barbarum polysaccharides were used to incubate with BIU87cells. LY-294002 and IGF-1 were used to inhibit and activate Pi3K/AKT pathway respectively. MTT were used to investigate the proliferation of BIU87cells. Transwell chambers and wound healing were used to test the migratory ability of BIU87cells. Western blotting were used to investigate the expressions of P21,P27,MMP-2, MMP-9, AKT and p-AKT in BIU87cells. Compared with the control group, the proliferation and migration of BIU87cells and the expression of p-AKT were significantly decreased in the study group; the inhibitory effect of the downregulation of p-AKT by LY-294002on the induction of BIU87cells proliferation and migration was identical to that of Lycium barbarum polysaccharides; upregulation of p-AKT by IGF-1 reversed the Lycium barbarum polysaccharides-induced inhibition of BIU87cells dedifferentiation. In conclusion, LBP inhibits the proliferation and migration of BIU87 cells by suppressing Pi3K/AKT signaling pathway.


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