Oncotarget

Research Papers: Gerotarget (Focus on Aging):

Influence of aging on the activity of mice Sca-1+CD31 cardiac stem cells

Qiong Wu, Jinxi Zhan, Shiming Pu, Liu Qin, Yun Li and Zuping Zhou _

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Oncotarget. 2017; 8:29-41. https://doi.org/10.18632/oncotarget.13930

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Abstract

Qiong Wu1,2,3,*, Jinxi Zhan1,2,3,*, Shiming Pu1,2,3, Liu Qin1,2,3, Yun Li1,2,3 and Zuping Zhou1,2,3

1 School of Life Sciences, Guangxi Normal University, Guilin, China

2 Guangxi Universities Key Laboratory of Stem cell and Biopharmaceutical Technology, Guangxi Normal University, Guilin, China

3 Research Center for Biomedical Sciences, Guangxi Normal University, Guilin, China

* These authors have contributed equally to this work

Correspondence to:

Zuping Zhou, email:

Keywords: cardiac resident stem/progenitor cells, aging, differentiation, proliferation, Gerotarget

Received: September 28, 2016 Accepted: November 24, 2016 Published: December 14, 2016

Abstract

Therapeutic application of cardiac resident stem/progenitor cells (CSC/CPCs) is limited due to decline of their regenerative potential with donor age. A variety of studies have shown that the cardiac aging was the problem of the stem cells, but little is known about the impact of age on the subgroups CSC/CPCs, the relationship between subgroups CSC/CPCs ageing and age-related dysfunction. Here, we studied Sca-1+CD31− subgroups of CSCs from younger(2~3months) and older(22~24months) age mice, biological differentiation was realized using specific mediums for 14 days to induce cardiomyocyte, smooth muscle cells or endothelial cells and immunostain analysis of differentiated cell resulting were done. Proliferation and cell cycle were measured by flow cytometry assay, then used microarray to dissect variability from younger and older mice. Although the number of CSCs was higher in older mice, the advanced age significantly reduced the differentiation ability into cardiac cell lineages and the proliferation ability. Transcriptional changes in Sca-1+CD31− subgroups of CSCs during aging are related to Vitamin B6 metabolism, circadian rhythm, Tyrosine metabolism, Complement and coagulation cascades. Taking together these results indicate that Cardiac resident stem/progenitor cells have significant differences in their proliferative, pluripotency and gene profiles and those differences are age depending.


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