TM4SF1 promotes the self-renewal of esophageal cancer stem-like cells and is regulated by miR-141
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Lei Xue1,*, Xiying Yu2,3,*, Xingran Jiang2,5, Xin Deng2,3, Linlin Mao2,3, Liping Guo2,3, Jinhu Fan4, Qinqxia Fan1, Liuxing Wang1, Shih-Hsin Lu2,3
1Department of Oncology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China
2Department of Etiology and Carcinogenesis and State Key Laboratory of Molecular Oncology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College (PUMC), Beijing, China
3Beijing Key Laboratory for Carcinogenesis and Cancer Prevention, Beijing, China
4Department of Cancer Epidemiology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College (PUMC), Beijing, China
5Current address: Department of Pathology, Beijing ChaoYang Hospital, Capital Medical University, Beijing, China
*These authors have contributed equally to this work
Liuxing Wang, email: [email protected]
Keywords: cancer stem-like cells, TM4SF1, miR-141, esophageal cancer, ESCC
Abbreviations: SP: side population, NSP: none side population, ESCC: esophageal squamous cell carcinoma, FTC: fumitremorgin C
Received: June 07, 2016 Accepted: November 22, 2016 Published: December 10, 2016
Cancer stem-like cells have been identified in primary human tumors and cancer cell lines. Previously we found TM4SF1 gene was highly expressed in side population (SP) cells from esophageal squamous cell carcinoma (ESCC) cell lines, but the role and underlying mechanism of TM4SF1 in ESCC remain unclear. In this study, we observed TM4SF1 was up-regulated but miR-141 was down-regulated in SP cells isolated from ESCC cell lines. TM4SF1 could stimulate the self-renewal ability and carcinogenicity of esophageal cancer stem-like cells, and promote cell invasion and migration. In miR-141 overexpression cells, the expression of TM4SF1 was significantly reduced. We also found that overexpression of miR-141 could abolish the self-renewal ability and carcinogenicity of esophageal cancer stem-like cells and decrease cell invasion and migration by suppressing TM4SF1. Consequently, TM4SF1 is a direct target gene of miR-141. The regulation of TM4SF1 by miR-141 may play an important role in controlling self-renewals of esophageal cancer stem-like cells. It may also promote the development of new therapeutic strategies and efficient drugs to target ESCC stem-like cells.
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