Oncotarget

Research Papers:

The neurotrophin Neuritin1 (cpg15) is involved in melanoma migration, attachment independent growth, and vascular mimicry

Anja Katrin Bosserhoff, Nadja Schneider, Lisa Ellmann, Lucie Heinzerling and Silke Kuphal _

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Oncotarget. 2017; 8:1117-1131. https://doi.org/10.18632/oncotarget.13585

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Abstract

Anja Katrin Bosserhoff1, Nadja Schneider1, Lisa Ellmann2, Lucie Heinzerling3, Silke Kuphal1

1Institute of Biochemistry (Emil-Fischer-Center), Friedrich Alexander University Erlangen-Nürnberg, Erlangen, 91054, Germany

2Institute for Functional Genomics, University Regensburg, Regensburg, 93053, Germany

3Institute of Dermatology, University Hospital Erlangen, Friedrich Alexander University Erlangen-Nürnberg, Erlangen, 91054, Germany

Correspondence to:

Silke Kuphal, email: [email protected]

Keywords: NRN1, melanoma, migration, biomarker, serum

Received: June 20, 2016    Accepted: November 07, 2016    Published: November 25, 2016

ABSTRACT

The neurotrophin Neuritin1 (NRN1; cpg15) belongs to the candidate plasticity gene (CPG) family and is expressed in postmitotic-differentiating neurons of the developmental nervous system and neuronal structures associated with plasticity in the brain of human adult.

Our newest findings document that NRN1 deregulation could contribute also to disease development and have impact on malignant melanoma. Our analyses displayed the over-expression of NRN1 in melanoma in vitro and in vivo, shown by immunohistochemistry and qRT-PCR on microdissected melanoma tissue; furthermore, soluble NRN1 was detectable in tissue culture supernatant and serum of melanoma patients.

To investigate the role of NRN1 in melanoma we performed knockdown, over-expression and recombinant-NRN1-treatment experiments affiliated by functional assays. Our results show that migration, attachment independent growth and vasculogenesis were affected after manipulation of NRN1 on endogenous and extrinsic level. Interestingly, high NRN1 serum levels correlate with low MIA serum levels (< 10ng/ml). Therefore, we speculate that NRN1 could be a marker for early melanoma stages, in particular.

In summary, we detected an overexpression of NRN1 in melanoma patient. In functional cell culture experiments we found a correlation between NRN1 expression and the cancerous behavior of melanoma cells.


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