Oncotarget

Research Papers:

MicroRNA-206 is involved in the pathogenesis of ulcerative colitis via regulation of adenosine A3 receptor

Weiyun Wu, Yanting He, Xiao Feng, Shicai Ye, Hao Wang, Wenkai Tan, Caiyuan Yu, Juxiang Hu, Rong Zheng and Yu Zhou _

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Oncotarget. 2017; 8:705-721. https://doi.org/10.18632/oncotarget.13525

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Abstract

Weiyun Wu1,*, Yanting He1,*, Xiao Feng1, Shicai Ye1, Hao Wang1, Wenkai Tan1, Caiyuan Yu1, Juxiang Hu1, Rong Zheng1, Yu Zhou1

1Department of Gastroenterology, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China

*These authors contributed equally to this work

Correspondence to:

Yu Zhou, email: ahdg2005@126.com

Keywords: miRNA-206, ulcerative colitis, adenosine A3 receptor, NF-κB

Received: June 09, 2016     Accepted: November 12, 2016     Published: November 23, 2016

ABSTRACT

Increasing evidence suggests that miRNAs are widely dysregulated in ulcerative colitis (UC), potentially affecting UC pathogenesis, diagnosis, and therapy. microRNA (miR) -206 has been reported to be upregulated in UC; however, its function and role in UC remain unknown. Here, we elucidate the function of miR-206 in the pathogenesis of UC. In patients with active-UC, miR-206 and adenosine A3 receptor (A3AR) levels were significantly upregulated and downregulated, respectively, and were inversely correlated. A3AR was expressed in the colon mucosa (particularly in colon epithelial-cell membranes). In HT-29 cells, miR-206 downregulated A3AR mRNA/protein expression by directly targeting the A3AR 3′-UTR; miR-206 overexpression and knockdown respectively increased and decreased TNF-α-induced nuclear NF-κB/p65, p-IκB-α, IKKα, p-IKKα and IL-8/IL-1β secretion. However, A3AR-siRNA reversed the miR-206 inhibitory effect. Furthermore, miR-206 increased dextran sodium sulphate-induced colitis severity (i.e., increased bodyweight loss, DAI score, colon shrinkage, and MPO activity), which was partially ameliorated by miR-206-antagomir treatment. miR-206-agomir treatment potently suppressed A3AR expression and increased NF-κB signalling and downstream cytokine (TNF-α/IL-8/IL-1β) expression in the mouse colon, in contrast to miR-206-antagomir administration. Taken together, our results demonstrated that miR-206 has a proinflammatory role in UC by downregulating A3AR expression and activating NF-κB signalling.


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