Tag SNPs of long non-coding RNA TINCR affect the genetic susceptibility to gastric cancer in a Chinese population
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Xiang Ma1,*, Chi Huang1,*, Dakui Luo1,*, Younan Wang1,*, Ran Tang1, Xiangkun Huan1, Yi Zhu2, Zekuan Xu1, Ping Liu3, Li Yang1
1Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China
2Jiangsu Province Academy of Clinical Medicine, Institute of Tumor Biology, Nanjing, China
3Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China
*These authors have contributed equally to this work
Li Yang, email: [email protected]
Keywords: gastric cancer, TINCR, polymorphism, genotype
Received: February 14, 2016 Accepted: November 06, 2016 Published: November 23, 2016
Tissue differentiation-inducing non-protein coding RNA (TINCR) is required for normal epidermal differentiation. TINCR is also strongly overexpressed in human gastric cancer (GC) and contributes to carcinogenesis and tumor progression. However, the association between TINCR polymorphisms and the risk of any diseases, such as GC, remains unknown. In the present study, the tag single nucleotide polymorphisms rs8113645, rs2288947, rs8105637, and rs12610531 were analyzed in 602 patients with GC and 602 age- and sex-matched controls. Polymorphisms were genotyped using TaqMan technology. Carriers of variant rs8113645 and rs2288947 alleles indicated reduced risks of GC (p = 0.003 and 0.037, respectively). A allele genotypes of rs8113645 and G allele genotypes of rs2288947 (rs8113645 GA and AA; rs2288947 AG and GG) were also significantly associated with decreased GC risk (p < 0.05). Stratification analysis displayed that the correlations between GC risk and variant genotypes of both rs8113645 and rs2288947were more evident in younger individuals, men, nonsmokers, and individuals from rural areas. We also demonstrated that rs8113645 GA+AA genotype carriers had lower TINCR mRNA expression levels compared with common genotype in both normal and GC tissues (p < 0.05). These results suggest that long non-coding RNA TINCR polymorphisms may be implicated in GC development.
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