Oncotarget

Research Papers: Pathology:

Variable patterns of ectopic mineralization in Enpp1asj-2J mice, a model for generalized arterial calcification of infancy

Sarah Y. Siu, Nathaniel A. Dyment, David W. Rowe, John P. Sundberg, Jouni Uitto and Qiaoli Li _

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Oncotarget. 2016; 7:83837-83842. https://doi.org/10.18632/oncotarget.13335

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Abstract

Sarah Y. Siu1, Nathaniel A. Dyment2, David W. Rowe2, John P. Sundberg3, Jouni Uitto1,4 and Qiaoli Li1,4

1 Department of Dermatology and Cutaneous Biology, The Sidney Kimmel Medical College and The PXE International Center of Excellence in Research and Clinical Care, Thomas Jefferson University, Philadelphia, PA, USA

2 Center for Regenerative Medicine and Skeletal Development, University of Connecticut Health Center, Farmington, CT, USA

3 The Jackson Laboratory, Bar Harbor, ME, USA

4 Jefferson Institute of Molecular Medicine, Thomas Jefferson University, Philadelphia, PA, USA

Correspondence to:

Qiaoli Li, email:

Keywords: ectopic mineralization; generalized arterial calcification of infancy; mouse models; cryohistology; Pathology Section

Received: October 07, 2016 Accepted: November 02, 2016 Published: November 14, 2016

Abstract

Generalized arterial calcification of infancy (GACI) is an autosomal recessive disorder characterized by early onset of extensive mineralization of the cardiovascular system. The classical forms of GACI are caused by mutations in the ENPP1 gene, encoding a membrane-bound pyrophosphatase/phosphodiesterase that hydrolyzes ATP to AMP and inorganic pyrophosphate. The asj-2J mouse harboring a spontaneous mutation in the Enpp1 gene has been characterized as a model for GACI. These mutant mice develop ectopic mineralization in skin and vascular connective tissues as well as in cartilage and collagen-rich tendons and ligaments. This study examined in detail the temporal ectopic mineralization phenotype of connective tissues in this mouse model, utilizing a novel cryo-histological method that does not require decalcification of bones. The wild type, heterozygous, and homozygous mice were administered fluorescent mineralization labels at 4 weeks (calcein), 10 weeks (alizarin complexone), and 11 weeks of age (demeclocycline). Twenty-four hours later, outer ears, muzzle skin, trachea, aorta, shoulders, and vertebrae were collected from these mice and examined for progression of mineralization. The results revealed differential timeline for disease initiation and progression in various tissues of this mouse model. It also highlights the advantages of cryo-histological fluorescent imaging technique to study mineral deposition in mouse models of ectopic mineralization disorders.


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