Research Papers:
miR-26a induced the suppression of tumor growth of cholangiocarcinoma via KRT19 approach
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Abstract
Ping Wang2, Long Lv1
1Department of General Surgery, People’s Hospital of Gaochun, Gaochun, Nanjing, 211300, Jiangsu Province, China
2Department of Endocrinology, People’s Hospital of Gaochun, Gaochun, Nanjing, 211300, Jiangsu Province, China
Correspondence to:
Long Lv, email: [email protected]
Keywords: proliferation, cholangiocarcinoma, stem cell, miRNA, KRT19
Received: July 18, 2016 Accepted: October 13, 2016 Published: November 09, 2016
ABSTRACT
Background and Aims: KRT19 was identified as one of the key biomarkers for distinguishing cholangiocarcinoma (CCA) and hepatocellular carcinoma. The detailed role of miRNAs involved in the oncogenic incident of KRT19 was poor investigated.
Results: Based on prediction and validation, miR-26a was inversely correlated with KRT19 in patients’ tissues samples and biopsies. Ectopic expression of miR-26a dramatically suppressed cell proliferation and tumor growth in vitro and in vivo. Knock-down miR-26a could induce an increasing population of SP cells by promoting KRT19 expression. The KRT19 was also suppressed via directly binding at 3′UTR region by miR-26a.
Materials and Methods: Bioinformatics prediction was first applied to screening the potential miRNA involved. RT-PCR, Immunohistochemistry and Western blot were used to examine the expression of miRNAs and candidate genes in 65 pairs of cholangiocarcinoma. The loss-and gain-function assay was employed to detect the role of certain miRNA in vitro and vivo. Side-population (SP) cells were detected and sorted by flow cytometry.
Conclusions: Aberrant decreased miR-26a could promote cell proliferation by regulating KRT19 which play important roles in the pathogenesis of CCA.
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PII: 13229