Oncotarget

Research Papers:

Subsite-specific association of DEAD box RNA helicase DDX60 with the development and prognosis of oral squamous cell carcinoma

Ting-Ying Fu _, Chao-Nan Wu, Huei-Cin Sie, Jiin-Tsuey Cheng, Yaoh-Shiang Lin, Huei-Han Liou, Yu-Kai Tseng, Chih-Wen Shu, Kuo-Wang Tsai, Leing-Ming Yen, Hui-Wen Tseng, Ching-Jiunn Tseng, Luo-Ping Ger and Pei-Feng Liu

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Oncotarget. 2016; 7:85097-85108. https://doi.org/10.18632/oncotarget.13197

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Abstract

Ting-Ying Fu1,2,*, Chao-Nan Wu3,4,*, Huei-Cin Sie1, Jiin-Tsuey Cheng5, Yaoh-Shiang Lin6, Huei-Han Liou7, Yu-Kai Tseng8,9, Chih-Wen Shu7, Kuo-Wang Tsai7,10, Leing-Ming Yen1, Hui-Wen Tseng11,12, Ching-Jiunn Tseng7,13,14,15, Luo-Ping Ger7,16, Pei-Feng Liu7,17

1Department of Pathology and Laboratory Medicine, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan

2Department of Optometry, Shu-Zen Junior College of Medicine and Management, Kaohsiung, Taiwan

3Department of Stomatology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan

4Department of Dental Technology, Shu-Zen Junior College of Medicine and Management, Kaohsiung, Taiwan

5Department of Biological Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan

6Department of Otorhinolaryngology-Head & Neck Surgery, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan

7Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan

8Department of Orthopedics, Show Chwan Memorial Hospital, Changhua, Taiwan

9Department of Orthopedics, National Cheng Kung University Hospital, Tainan, Taiwan

10Department of Chemical Biology, National Pingtung University of Education, Pingtung, Taiwan

11Department of Dermatology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan

12Department of Nursing, Meiho University, Pingtung, Taiwan

13Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan

14Department of Pharmacology, National Defense Medical Center, Taipei, Taiwan

15Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan

16Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan

17Department of Biotechnology, Fooyin University, Kaohsiung, Taiwan

*These authors have contributed equally to this work

Correspondence to:

Luo-Ping Ger, email: lpger@vghks.gov.tw

Pei-Feng Liu, email: pfliu@vghks.gov.tw

Keywords: DDX60, oral cancer, subsite-specific, tumorigenesis, prognosis

Received: April 14, 2016     Accepted: October 28, 2016     Published: November 08, 2016

ABSTRACT

The clinical significance and biological function of DEXD/H box helicase 60 (DDX60) in oral cancer remains unknown. Herein, we evaluated the association of DDX60 expression with tumorigenesis and the prognosis of oral squamous cell carcinoma (OSCC). DDX60 expression was examined by immunohistochemistry on tissue microarray slides of 494 OSCC patients, including 180 buccal mucosal SCC (BMSCC), 241 tongue SCC (TSCC), and 73 lip SCC (LSCC) patients. DDX60 expression was significantly increased in all three subsites of OSCC compared to its expression in tumor adjacent normal tissues. However, its association with tumorigenesis was specific to the oral cavity subsite after the stratification of betel quid chewing, smoking, and drinking. Among OSCC patients, higher levels of DDX60 expression were associated with the male gender, a well-differentiated tumor, advanced stage of disease, and a large tumor size with subsite specific features. LSCC patients with high DDX60 expression levels showed shorter disease-specific survival, particularly those with moderately or poorly differentiated tumors. Additionally, TSCC or OSCC patients with high DDX60 expression showed a poor disease-free survival (DFS), particularly those with moderately or poorly differentiated tumors. Therefore, DDX60 is a novel and unfavorable biomarker for tumorigenesis and prognosis of OSCC in a subsite-specific manner.


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