Advance Publications: Research Papers:
Triptolide contributes to decrease in TLR4 expression by upregulating miR-224-3p to inhibit the inflammatory reaction in diabetic nephropathy
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Bei Sun1,*, Shaolan Hu1,*, Yongmei Li2, Endong Zhu1, Li Li1, Fei Han1, Chun-Jun Li1 and Liming Chen1
1 Key Laboratory of Hormones and Development, Ministry of Health, Tianjin Key Laboratory of Metabolic Diseases, Tianjin Metabolic Diseases Hospital & Tianjin Institute of Endocrinology, Tianjin Medical University, Tianjin, China
2 Department of Medical Microbiology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China
* These authors have contributed equally to this work
Liming Chen, email:
Chun-Jun Li, email:
Keywords: diabetic nephropathy, triptolide, miRNA, TLR4, inflammation, Pathology Section
Received: August 01, 2016 Accepted: October 13, 2016 Published: November 04, 2016
Triptolide has been shown to have anti-inflammatory properties. miRNAs have emerged as important regulators of DN. However, the role of miRNAs in inflammation regulation during DN development is poorly understood. In this study, we investigated the effects of the association between miRNAs and triptolide on inflammation in DN. We found that triptolide significantly inhibits the inflammatory reactions of DN in rats, and miR-224-3p is selectively upregulated more than 2 fold in NRK-52E cells treated with triptolide compared to the cells without treatment. miR-224-3p overexpression exerts similar effects to those of triptolide, while miR-224-3p downregulation increases all the inflammatory reactions above. In summary, we found that triptolide attenuates the inflammatory reactions in DN by upregulating miR-224-3p expression.
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