Research Papers:
CDH4 suppresses the progression of salivary adenoid cystic carcinoma via Ecadherin coexpression
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Jian Xie1,*, Yan Feng1,*, Ting Lin1,*, Xiao-Yu Huang1, Rui-Huan Gan1, Yong Zhao2, Bo-Hua Su1, Lin-Can Ding1, Lin She1, Jiang Chen3, Li-Song Lin4, Xu Lin5, Da-Li Zheng5, You-Guang Lu1
1Department of Preventive Dentistry, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, China
2Department of Pathology, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, China
3Center of Dental Implant, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, China
4Department of Oral and Maxillofacial Surgery, Affiliated First Hospital of Fujian Medical University, Fuzhou, China
5Key Laboratory of Ministry of Education for Gastrointestinal Cancer, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, China
*These authors have contributed equally to this work
Correspondence to:
Da-Li Zheng, email: [email protected]
You-Guang Lu, email: [email protected]
Keywords: CDH4, salivary adenoid cystic carcinoma, CDH1, proliferation, invasion
Received: May 23, 2016 Accepted: October 14, 2016 Published: October 22, 2016
ABSTRACT
The cadherin-4 gene (CDH4) of the cadherin family encodes non-epithelial R-cadherin (R-cad); however, the function of this gene in different types of cancer remains controversial. In this study, we found higher expression of CDH4 mRNA in a salivary adenoid cystic carcinoma (SACC) cell line with low metastatic potential (SACC-83) than in a cell line with high metastatic potential (SACC-LM). By analyzing 67 samples of SACC tissues and 40 samples of paraneoplastic normal tissues, we found R-cad highly expressed in 100% of normal paraneoplastic tissue but only expressed in 64% of SACC tumor tissues (P<0.001). Knockdown of CDH4 expression in vitro promoted the growth, mobility and invasion of SACC cells, and in vivo experiments showed that decreased CDH4 expression enhanced SACC tumorigenicity. Furthermore, CDH4 suppression resulted in down-regulation of E-cadherin (E-cad), which is encoded by CDH1 gene and is a well-known tumor suppressor gene by inhibition of cell proliferation and migration. These results indicate that CDH4 may play a negative role in the growth and metastasis of SACC via co-expression with E-cadherin.