MiR-384 inhibits human colorectal cancer metastasis by targeting KRAS and CDC42
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Yong-Xia Wang1,2,3,4,*, Yan-Ru Chen1,2,3,*, Shan-Shan Liu1,2,3,*, Ya-Ping Ye1,2,3, Hong-Li Jiao1,2,3, Shu-Yang Wang1,2,3, Zhi-Yuan Xiao1,2,3, Wen-Ting Wei1,2,3, Jun-Feng Qiu1,2,3, Li Liang1,2,3, Wen-Ting Liao1,2,3, Yan-Qing Ding1,2,3
1Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China
2Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China
3Guangdong Province Key Laboratory of Molecular Tumor Pathology, Guangzhou, Guangdong, China
4Department of Pathology, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan, China
*These authors have contributed equally to this work
Wen-Ting Liao: email: [email protected]
Yan-Qing Ding: email: [email protected]
Keywords: colorectal cancer, MiR-384, metastasis, KRAS, CDC42
Received: July 20, 2016 Accepted: October 03, 2016 Published: October 17, 2016
Colorectal cancer (CRC) is the third most common cancer worldwide. Metastatic progression is a primary factor contributing to lethality of CRC patients. However, the molecular mechanisms forming early local invasion and distant metastatic colonies are still unclear and the present therapeutic approaches for CRC are unsatisfactory. Therefore, novel therapies targeting metastatic invasion that could prevent tumor spreading and recurrence are urgently needed. Our study showed that the decrease of miR-384 was found in 83.0% (83/100) CRC patients. And low-leveled expression of miR-384 was closely correlated with the invasive depth, lymph node and distant metastasis of CRC. Overexpression of miR-384 could inhibit the invasive and migrating abilities of CRC cells in vitro and the metastatic potential in vivo. Luciferase assays showed that miR-384 repressed the expression of Kirsten Ras (KRAS) and Cell division cycle 42 (CDC42) by directly targeting their 3’-untranslated regions. There is functional and mechanistic relationship between miRNA-384 and KRAS, CDC42 in the invasion and metastasis of CRC. And our findings suggest that miR-384could be a potent therapeutic target for CRC. Restoration of miR-384 expression might provide novel therapeutic approach to the reduction of CRC metastasis.
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