Oncotarget

Research Papers:

MicroRNA-7 inhibits cell proliferation, migration and invasion in human non-small cell lung cancer cells by targeting FAK through ERK/MAPK signaling pathway

Qi Cao, Zheng-Dao Mao, Yu-Jia Shi, Yi Chen, Yun Sun, Qian Zhang _, Lei Song and Li-Ping Peng

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Oncotarget. 2016; 7:77468-77481. https://doi.org/10.18632/oncotarget.12684

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Abstract

Qi Cao1,*, Zheng-Dao Mao1,*, Yu-Jia Shi1, Yi Chen1, Yun Sun1, Qian Zhang1, Lei Song1, Li-Ping Peng1

1Department of Respiratory Medicine, Affiliated Changzhou No.2 People’s Hospital, Nanjing Medical University, Changzhou 213003, P.R. China

*This authors have contributed equally to this work

Correspondence to:

Qian Zhang, email: [email protected]

Keywords: non-small cell lung cancer, microRNA-7, FAK, ERK/MAPK signaling pathway, cell proliferation

Received: April 23, 2016    Accepted: September 02, 2016    Published: October 15, 2016

ABSTRACT

Objective: To investigate the effects of microRNA-7 (miR-7) on the proliferation, migration and invasion of non-small cell lung cancer NSCLC) cells by targeting FAK through ERK/MAPK signaling pathway.

Methods: NSCLC tissues and adjacent normal tissues were obtained from 160 NSCLC patients after operation. NSCLC cell lines (A549, H1299 and H1355) and a normal human fetal lung fibroblast cell line (MRC-5) were obtained. NSCLC cells were assigned into miR-7 inhibitors, miR-7 mimics, blank, miR-7 mimics control, miR-7 inhibitors control, FAK siRNA and miR-7 inhibitors + FAK siRNA groups. The expressions of miR-7 and FAK mRNA in tissues and cell lines were detected by qRT-PCR and Western-Blotting. Cell proliferation, migration and invasion were detected by MTT assay, wound scratch assay and Transwell assay.

Results: Compared with adjacent normal tissues, miR-7 expression was down-regulated, but the mRNA and protein expressions of FAK, ERK and MAPK were up-regulated. Compared with the blank and mimics control groups, miR-7 significantly increased but FAK, ERK and MAPK expressions decreased in miR-7 mimics and FAK siRNA groups. Cell proliferation, migration and invasion were inhibited in the miR-7 mimics and FAK siRNA groups, while opposite regarding miR-7 inhibitors group.

Conclusion: The miR-7 can inhibit the activation of ERK/MAPK signaling pathway by down-regulating FAK expression, thereby suppressing the proliferation, migration and invasion of NSCLC cells. The miR-7 and its target gene FAK may be novel targets for the diagnosis and treatment of NSCLC.


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