Research Papers:

miR-885-5p suppresses hepatocellular carcinoma metastasis and inhibits Wnt/β-catenin signaling pathway

Zhuhong Zhang, Jing Yin, Jian Yang, Wenzhi Shen, Chunyan Zhang, Wenjun Mou, Jinhua Luo, Hua Yan, Peiqing Sun, Yunping Luo, Yaping Tian and Rong Xiang _

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Oncotarget. 2016; 7:75038-75051. https://doi.org/10.18632/oncotarget.12602

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Zhuhong Zhang1,3,6, Jing Yin1, Jian Yang1, Wenzhi Shen1, Chunyan Zhang1,2, Wenjun Mou1,2, Jinhua Luo1,2, Hua Yan3, Peiqing Sun4, Yunping Luo5, Yaping Tian2, Rong Xiang1

1Department of Immunology, School of Medicine, Nankai University, Tianjin, 300071, China

2Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing, 100853, China

3Department of Ophthalmology, Tianjin Medical University General Hospital, Tianjin, 300052, China

4The Scripps Research Institute, La Jolla, CA, 92037, USA

5Department of Immunology, Institute of Basic Medical Science, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, 100005, China

6Current address: Division of Genetic and Molecular Toxicology, National Center for Toxicological Research, FDA, Jefferson, AR 72079, USA

Correspondence to:

Rong Xiang, email: [email protected]

Yaping Tian, email: [email protected], [email protected]

Keywords: miR-885-5p, Wnt/β-catenin HCC, migration, invasion

Received: June 14, 2016     Accepted: September 25, 2016     Published: October 12, 2016


MicroRNAs (miRNAs) inhibit or improve the malignant progression of hepatocellular carcinoma (HCC). We previously reported that compared to health controls, patients with liver cirrhosis present the highest levels of circulating miR-885-5p, followed by those with chronic hepatitis B and those with HCC. However, the molecular involvement of miR-885-5p in HCC metastasis is presently unclear. Here, we demonstrated that the expression of miR-885-5p negatively correlated with the invasive and metastatic capabilities of human HCC tissue samples and cell lines. We found that miR-885-5p expression levels correlated with the survival of patients with HCC. Overexpression of miR-885-5p decreased metastasis of HCC cells in vitro and in vivo. Inhibition of miR-885-5p improved proliferation of non-metastatic HCC cells. Furthermore, we disclosed that miR-885-5p targeted gene encoding β-catenin CTNNB1, leading to decreased activity of the Wnt/β-catenin signaling pathway. The present study indicates that miR-885-5p suppresses the metastasis of HCC and inhibits Wnt/β-catenin signaling pathway by its CTNNB1 target, which suggests that miR-885-5p to be a promising negative regulator of HCC progression and as a novel therapeutic agent to treat HCC.

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