Research Papers:
miR8855p suppresses hepatocellular carcinoma metastasis and inhibits Wnt/βcatenin signaling pathway
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Zhuhong Zhang1,3,6, Jing Yin1, Jian Yang1, Wenzhi Shen1, Chunyan Zhang1,2, Wenjun Mou1,2, Jinhua Luo1,2, Hua Yan3, Peiqing Sun4, Yunping Luo5, Yaping Tian2, Rong Xiang1
1Department of Immunology, School of Medicine, Nankai University, Tianjin, 300071, China
2Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing, 100853, China
3Department of Ophthalmology, Tianjin Medical University General Hospital, Tianjin, 300052, China
4The Scripps Research Institute, La Jolla, CA, 92037, USA
5Department of Immunology, Institute of Basic Medical Science, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, 100005, China
6Current address: Division of Genetic and Molecular Toxicology, National Center for Toxicological Research, FDA, Jefferson, AR 72079, USA
Correspondence to:
Rong Xiang, email: [email protected]
Yaping Tian, email: [email protected], [email protected]
Keywords: miR-885-5p, Wnt/β-catenin HCC, migration, invasion
Received: June 14, 2016 Accepted: September 25, 2016 Published: October 12, 2016
ABSTRACT
MicroRNAs (miRNAs) inhibit or improve the malignant progression of hepatocellular carcinoma (HCC). We previously reported that compared to health controls, patients with liver cirrhosis present the highest levels of circulating miR-885-5p, followed by those with chronic hepatitis B and those with HCC. However, the molecular involvement of miR-885-5p in HCC metastasis is presently unclear. Here, we demonstrated that the expression of miR-885-5p negatively correlated with the invasive and metastatic capabilities of human HCC tissue samples and cell lines. We found that miR-885-5p expression levels correlated with the survival of patients with HCC. Overexpression of miR-885-5p decreased metastasis of HCC cells in vitro and in vivo. Inhibition of miR-885-5p improved proliferation of non-metastatic HCC cells. Furthermore, we disclosed that miR-885-5p targeted gene encoding β-catenin CTNNB1, leading to decreased activity of the Wnt/β-catenin signaling pathway. The present study indicates that miR-885-5p suppresses the metastasis of HCC and inhibits Wnt/β-catenin signaling pathway by its CTNNB1 target, which suggests that miR-885-5p to be a promising negative regulator of HCC progression and as a novel therapeutic agent to treat HCC.