Research Papers:

The first investigation of Wilms’ tumour atomic structure-nitrogen and carbon isotopic composition as a novel biomarker for the most individual approach in cancer disease

Katarzyna Taran _, Tomasz Frączek, Anita Sikora-Szubert, Anna Sitkiewicz, Wojciech Młynarski, Józef Kobos and Piotr Paneth

PDF  |  HTML  |  How to cite

Oncotarget. 2016; 7:76726-76734. https://doi.org/10.18632/oncotarget.12521

Metrics: PDF 1529 views  |   HTML 1968 views  |   ?  


Katarzyna Taran1, Tomasz Frączek2, Anita Sikora-Szubert3, Anna Sitkiewicz4, Wojciech Młynarski5, Józef Kobos6, Piotr Paneth2

1Department of Pathology, Medical University of Lodz, Poland

2Institute of Applied Radiation Chemistry, Lodz University of Technology, Poland

3Clinic of High Risk Pregnancy, Medical University of Lodz, Poland

4Department of Oncology and Paediatric Surgery, Konopnicka Memorial Hospital, Medical University of Lodz, Poland

5Department of Pediatrics, Oncology, Hematology and Diabetology, Konopnicka Memorial Hospital, Medical University of Lodz, Poland

6Department of Paediatric Pathology, Medical University of Lodz, Poland

Correspondence to:

Katarzyna Taran, email: [email protected]

Keywords: Wilms’ tumour, stable isotopes, prognosis, anaplasia, personalized medicine

Received: June 09, 2016     Accepted: October 03, 2016     Published: October 08, 2016


The paper describes a novel approach to investigating Wilms’ tumour (nephroblastoma) biology at the atomic level. Isotope Ratio Mass Spectrometry (IRMS) was used to directly assess the isotope ratios of nitrogen and carbon in 84 Wilms’ tumour tissue samples from 28 cases representing the histological spectrum of nephroblastoma. Marked differences in nitrogen and carbon isotope ratios were found between nephroblastoma histological types and along the course of cancer disease, with a breakout in isotope ratio of the examined elements in tumour tissue found between stages 2 and 3. Different isotopic compositions with regard to nitrogen and carbon content were observed in blastemal Wilms’ tumour, with and without focal anaplasia, and in poorly- and well-differentiated epithelial nephroblastoma. This first assessment of nitrogen and carbon isotope ratio reveals the previously unknown part of Wilms’ tumour biology and represents a potential novel biomarker, allowing for a highly individual approach to treating cancer. Furthermore, this method of estimating isotopic composition appears to be the most sensitive tool yet for cancer tissue evaluation, and a valuable complement to established cancer study methods with prospective clinical impact.

Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
PII: 12521