New strategy to rescue the inhibition of osteogenesis of human bone marrow-derived mesenchymal stem cells under oxidative stress: combination of vitamin C and graphene foams
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Zubin Zhou1,*, Zhengliang Xu1,*, Feng Wang1, Ye Lu1, Peipei Yin1, Chaolai Jiang1, Yingjie Liu1, Hua Li2, Xiaowei Yu1, Yuqiang Sun1
1Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, China
2State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China
*These authors contributed equally to this work
Xiaowei Yu, email: [email protected]
Yuqiang Sun, email: [email protected]
Keywords: osteogenesis, mesenchymal stem cell, oxidative stress, vitamin C, graphene foams
Received: July 18, 2016 Accepted: September 29, 2016 Published: October 04, 2016
To rescue the oxidative stress induced inhibition of osteogenesis, vitamin C (VC) was chemically modified onto three-dimensional graphene foams (3D GFs), then their regulation on osteogenesis of human bone marrow-derived mesenchymal stem cells (BM-MSCs) was studied. Combined action of VC + GF significantly decreased H2O2-induced oxidative stress, and rescued H2O2-inhibited cell viability, differentiation and osteogenesis of BM-MSCs in vitro. Further studies revealed that Wnt pathway may be involved in this protection of osteogenesis. Furthermore, an in vivo mouse model of BM-MSCs transplantation showed that VC + GF remarkably rescued oxidative stress inhibited calcium content and bone formation. The combination of VC and GF exhibited more pronounced protective effects against oxidative stress induced inhibition of osteogenesis, compared to monotherapy of VC or GF. Our study proposed a new strategy in stem cell-based therapies for treating bone diseases.
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