Oncotarget

Research Papers:

MFAP5 and TNNC1: Potential markers for predicting occult cervical lymphatic metastasis and prognosis in early stage tongue cancer

Xi Yang, Kailiu Wu, Siyi Li, Longwei Hu, Jing Han, Dongwang Zhu, Xuerui Tian, Wei Liu, Zhen Tian, Laiping Zhong, Ming Yan, Chenping Zhang _ and Zhiyuan Zhang

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Oncotarget. 2017; 8:2525-2535. https://doi.org/10.18632/oncotarget.12446

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Abstract

Xi Yang1, Kailiu Wu1, Siyi Li1, Longwei Hu1, Jing Han1, Dongwang Zhu1, Xuerui Tian1, Wei Liu1, Zhen Tian2, Laiping Zhong1, Ming Yan1, Chenping Zhang1, Zhiyuan Zhang1

1Department of Oral & Maxillofacial - Head & Neck Oncology, Shanghai Key Laboratory of Stomatology, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China

2Department of Oral Pathology, Shanghai Key Laboratory of Stomatology, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China

Correspondence to:

Chenping Zhang, email: [email protected]

Zhiyuan Zhang, email: [email protected]

Keywords: potential marker, metastasis and prognosis, tongue cancer, microarray

Received: May 30, 2016     Accepted: September 20, 2016     Published: October 04, 2016

ABSTRACT

The purpose of this study is to identify candidate genes that could predict prognosis of early-stage tongue squamous cell carcinoma (TSCC) and its occult cervical lymphatic metastasis by large-scale gene expression profiling. Tumor tissue and matched normal mucosa samples were collected from patients with TSCC and analyzed with Affymetrix HTA2.0 high-density oligonucleotide array. Differentially expressed genes in TSCC with cervical lymph node metastasis (CLNM) were further analyzed with Gene Ontology and Kyoto Encyclopedia of Genes and Genomes for their functions and related pathways. A total of 107 differentially expressed genes (p < 0.05) were identified by microarray in TSCC samples with CLNM (n = 6) compared to those without CLNM (n = 6). Genes involved in the cell-matrix adherens junction and migration function including MFAP5, TNNC1, MGP, FBFBP1 and FBXO32 were selected and validated by RT-PCR in TSCC samples (n = 32). Of the five genes, MFAP5 and TNCC1 expressions were further validated by immohistochemistry (n = 61). The significant positive correlation between MFAP5 and TNNC1 expression (p<0.001) was observed. Notably, over-expression of MFAP5 and TNNC1 were correlated with CLNM, metastasis relapse-free survival and overall survival. Our findings indicated that MFAP5 and TNNC1 may be potential markers for predicting occult cervical lymphatic metastasis and prognosis of oral tongue carcinoma.


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