Oncotarget

Research Papers:

Age and cellular context influence rectal prolapse formation in mice with caecal wall colorectal cancer xenografts

Joke Tommelein, Félix Gremonprez, Laurine Verset, Elly De Vlieghere, Glenn Wagemans, Christian Gespach, Tom Boterberg, Pieter Demetter, Wim Ceelen, Marc Bracke and Olivier De Wever _

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Oncotarget. 2016; 7:75603-75615. https://doi.org/10.18632/oncotarget.12312

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Abstract

Joke Tommelein1,2, Félix Gremonprez2,3, Laurine Verset4, Elly De Vlieghere1,2, Glenn Wagemans1,2, Christian Gespach5, Tom Boterberg1,2, Pieter Demetter4, Wim Ceelen2,3, Marc Bracke1,2, Olivier De Wever1,2

1Laboratory of Experimental Cancer Research, Department of Radiation Oncology and Experimental Cancer Research, Ghent University, Ghent, Belgium

2Cancer Research Institute Ghent (CRIG), Ghent, Belgium

3Department of Surgery, Ghent University Hospital, Ghent, Belgium

4Department of Pathology, Erasme University Hospital, Université Libre de Bruxelles, Brussels, Belgium

5Institut National de la Santé et de la Recherche Médicale, INSERM, Department of Molecular and Clinical Oncology, Université Paris VI Pierre et Marie Curie, Paris, France

Correspondence to:

Olivier De Wever, email: olivier.dewever@ugent.be

Keywords: rectal prolapse, COLO320DM, colorectal cancer, orthotopic, mouse model

Received: May 03, 2016     Accepted: September 14, 2016     Published: September 28, 2016

ABSTRACT

In patients with rectal prolapse is the prevalence of colorectal cancer increased, suggesting that a colorectal tumor may induce rectal prolapse. Establishment of tumor xenografts in immunodeficient mice after orthotopic inoculations of human colorectal cancer cells into the caecal wall is a widely used approach for the study of human colorectal cancer progression and preclinical evaluation of therapeutics. Remarkably, 70% of young mice carrying a COLO320DM caecal tumor showed symptoms of intussusception of the large bowel associated with intestinal lumen obstruction and rectal prolapse. The quantity of the COLO320DM bioluminescent signal of the first three weeks post-inoculation predicts prolapse in young mice. Rectal prolapse was not observed in adult mice carrying a COLO320DM caecal tumor or young mice carrying a HT29 caecal tumor. In contrast to HT29 tumors, which showed local invasion and metastasis, COLO320DM tumors demonstrated a non-invasive tumor with pushing borders without presence of metastasis. In conclusion, rectal prolapse can be linked to a non-invasive, space-occupying COLO320DM tumor in the gastrointestinal tract of young immunodeficient mice. These data reveal a model that can clarify the association of patients showing rectal prolapse with colorectal cancer.


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