Oncotarget

Research Papers:

X-radiation enhances the collagen type I strap formation and migration potentials of colon cancer cells

Stephanie Blockhuys, Na Liu, Nisha Rani Agarwal, Annika Enejder, Vesa Loitto and Xiao-Feng Sun _

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Oncotarget. 2016; 7:71390-71399. https://doi.org/10.18632/oncotarget.12111

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Abstract

Stephanie Blockhuys1,3, Na Liu1, Nisha Rani Agarwal3, Annika Enejder3, Vesa Loitto2, Xiao-Feng Sun1

1Department of Oncology and Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden

2Medical Microbiology, Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden

3Molecular Microscopy, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden

Correspondence to:

Xiao-Feng Sun, email: xiao-feng.sun@liu.se

Stephanie Blockhuys, email: steblo@chalmers.se

Keywords: collagen type 1, colorectal cancer, X-radiation, cell migration, integrin β1

Received: March 17, 2016     Accepted: September 13, 2016     Published: September 19, 2016

ABSTRACT

Rectal cancer treatment still fails with local and distant relapses of the disease. It is hypothesized that radiotherapy could stimulate cancer cell dissemination and metastasis. In this study, we evaluated the effect of X-radiation on collagen type I strap formation potential, i.e. matrix remodeling associated with mesenchymal cell migration, and behaviors of SW480, SW620, HCT116 p53+/+ and HCT116 p53−/− colon cancer cells. We determined a radiation-induced increase in collagen type I strap formation and migration potentials of SW480 and HCT116 p53+/+. Further studies with HCT116 p53+/+, indicated that after X-radiation strap forming cells have an increased motility. More, we detected a decrease in adhesion potential and mature integrin β1 expression, but no change in non-muscle myosin II expression for HCT116 p53+/+ after X-radiation. Integrin β1 neutralization resulted in a decreased cell adhesion and collagen type I strap formation in both sham and X-radiated conditions. Our study indicates collagen type I strap formation as a potential mechanism of colon cancer cells with increased migration potential after X-radiation, and suggests that other molecules than integrin β1 and non-muscle myosin II are responsible for the radiation-induced collagen type I strap formation potential of colon cancer cells. This work encourages further molecular investigation of radiation-induced migration to improve rectal cancer treatment outcome.


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