Quantitative proteomics reveals FLNC as a potential progression marker for the development of hepatocellular carcinoma
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Yingzi Qi1,*, Feng Xu1,*, Lingsheng Chen1,3, Yanchang Li1, Zhongwei Xu1, Yao Zhang1,7, Wei Wei1, Na Su1, Tao Zhang1, Fengxu Fan1,4, Xing Wang1, Xue Qin6, Lingqiang Zhang1, Yinkun Liu5, Ping Xu1,2,4
1State Key Laboratory of Proteomics, National Center for Protein Sciences Beijing, Beijing Proteome Research Center, Institute of Radiation Medicine, Beijing 102206, China
2Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education and Wuhan University School of Pharmaceutical Sciences, Wuhan 430072, China
3State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530005, China
4Anhui Medical University, Hefei 230032, China
5Cancer Research Center, Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China
6Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China
7Institute of Microbiology, Chinese Academy of Science, Beijing 100101, China
*These authors contributed equally to this work
Ping Xu, email: [email protected]
Yinkun Liu, email: [email protected]
Keywords: hepatocellular carcinoma (HCC), iTRAQ, filamin C (FLNC)
Received: March 06, 2016 Accepted: September 02, 2016 Published: September 09, 2016
Hepatocellular carcinoma (HCC) caused by hepatitis B virus (HBV) infection is one of the most life-threatening human cancers in China. However, the pathogenesis of HCC development is still unclear. Here, we systemically analyzed liver tissues from different stages of HCC patients through 8-plex Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) approach. A total of 4,620 proteins were identified and 3,781 proteins were quantified. When T1, T2 and T3 tumor tissues were compared with T1 non-tumor cells, 330, 365 and 387 differentially expressed proteins were identified respectively. IPA (Ingenuity Pathway Analysis) analysis revealed that these differentially expressed proteins were involved in endothelial cancer, cell spreading, cell adhesion and cell movement of tumor cell lines pathway and so on. Further study showed that the filamin C (FLNC) protein was significantly overexpressed with the development of HCC, which might play an important role in HCC invasion and metastasis. These results were also confirmed with western blot (WB). The mRNA levels were significantly increased in 50 pairs of tumor and adjacent non-tumor tissues from TCGA database. The higher expression of FLNC in HCC might be a common phenomenon, thereby shedding new light on molecular mechanism and biomarker for the diagnosis purpose of HCC development.
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