Oncotarget

Research Papers:

The somatic POLE P286R mutation defines a unique subclass of colorectal cancer featuring hypermutation, representing a potential genomic biomarker for immunotherapy

Sung-Min Ahn, Adnan Ahmad Ansari, Jihun Kim, Deokhoon Kim, Sung-Min Chun, Jiyun Kim, Tae Won Kim, Inja Park, Chang-Sik Yu and Se Jin Jang _

PDF  |  HTML  |  Supplementary Files  |  How to cite

Oncotarget. 2016; 7:68638-68649. https://doi.org/10.18632/oncotarget.11862

Metrics: PDF 3253 views  |   HTML 4959 views  |   ?  


Abstract

Sung-Min Ahn1,2,*, Adnan Ahmad Ansari3,4,*, Jihun Kim4,5, Deokhoon Kim4, Sung-Min Chun4,5, Jiyun Kim5, Tae Won Kim6, Inja Park7, Chang-Sik Yu7, Se Jin Jang4,5

1Division of Hematology-Oncology, Department of Internal Medicine, Gachon University Gil Hospital, Incheon, South Korea

2Gachon Institute of Genome Medicine and Science, Gachon University Gil Hospital, Incheon, South Korea

3Department of Biomedical Engineering, University of Ulsan College of Medicine, Seoul, South Korea

4Asan Center for Cancer Genome Discovery, Asan Institute for Life Sciences, Asan Medical Center, Seoul, South Korea

5Department of Pathology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, South Korea

6Department of Oncology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, South Korea

7Department of Surgery, University of Ulsan College of Medicine, Asan Medical Center, Seoul, South Korea

*These authors have contributed equally to this work

Correspondence to:

Se Jin Jang, email: [email protected]

Chang-Sik Yu, email: [email protected]

Keywords: early-onset colorectal cancer, POLE, hypermutation, immunotherapy, PD-1 blockade

Received: March 30, 2016    Accepted: August 24, 2016    Published: September 06, 2016

ABSTRACT

Early-onset colorectal cancers (EOCRCs) may have biological or genomic features distinct from late-onset CRCs (LOCRCs). Previous studies have mostly focused on the germline predisposition conditions of EOCRCs, but we hypothesized that EOCRCs may have distinct somatic aberrations that accelerate cancer development. To identify the somatic aberrations that accelerate cancer development at an early age, we conducted whole exome sequencing for 28 polyposis-unrelated, microsatellite stable (MSS) EOCRCs with no known germline predisposition conditions. Surprisingly, we found two distinct groups in the context of mutational burden: 6 hypermutated cases with 2325 to 10973 mutations and 22 nonhypermutated cases with 47 to 154 mutations. Further analysis revealed that four of the six hypermutated cases had the same POLE P286R mutation. We validated this finding in 83 MSS EOCRCs and 27 MSS LOCRCs, which revealed that 7.2% of EOCRCs (6/83) had the POLE P286R mutation, which was not found in LOCRCs. Clinicopathologically, EOCRCs with POLE mutations occurred far more frequently in the right colon than in the left colon, affecting men more frequently than women. In summary, we have identified a unique subclass of colon cancer characterized by a hypermutation associated with the POLE mutation. The acquisition of the POLE mutation leading to hypermutation can accelerate cancer development. Clinically, this subset with hypermutation may be susceptible to immune checkpoint blockade.


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
PII: 11862