Oncotarget

Research Papers:

FAM65B controls the proliferation of transformed and primary T cells

Jeanne Froehlich, Margaux Versapuech, Laura Megrelis, Quitterie Largeteau, Sylvain Meunier, Corinne Tanchot, Georges Bismuth, Jérôme Delon and Marianne Mangeney _

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Oncotarget. 2016; 7:63215-63225. https://doi.org/10.18632/oncotarget.11438

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Abstract

Jeanne Froehlich1,2,3, Margaux Versapuech1,2,3, Laura Megrelis1,2,3, Quitterie Largeteau1,2,3, Sylvain Meunier4, Corinne Tanchot4, Georges Bismuth1,2,3, Jérôme Delon1,2,3,*, Marianne Mangeney1,2,3,*

1Inserm, Institut Cochin, Paris, France

2Cnrs, Paris, France

3Université Paris Descartes, Sorbonne Paris Cité, Paris, France

4Inserm, PARCC, Université Paris Descartes, Sorbonne Paris Cité, Paris, France

*These authors have contributed equally to this work

Correspondence to:

Marianne Mangeney, email: [email protected]

Keywords: cell proliferation, cell cycle, signaling, T lymphocytes, leukemia

Received: April 13, 2016     Accepted: August 10, 2016     Published: August 20, 2016

ABSTRACT

Cell quiescence is controlled by regulated genome-encoded programs that actively express genes which are often down-regulated or inactivated in transformed cells. Among them is FoxO1, a transcription factor that imposes quiescence in several cell types, including T lymphocytes. In these cells, the FAM65B encoding gene is a major target of FOXO1. Here, we show that forced expression of FAM65B in transformed cells blocks their mitosis because of a defect of the mitotic spindle, leading to G2 cell cycle arrest and apoptosis. Upon cell proliferation arrest, FAM65B is engaged in a complex containing two proteins well known to be involved in cell proliferation i.e. the HDAC6 deacetylase and the 14.3.3 scaffolding protein. In primary T cells, FAM65B is down-regulated upon T cell receptor engagement, and maintaining its expression blocks their proliferation, establishing that the decrease of FAM65B expression is required for proliferation. Conversely, inhibiting FAM65B expression in naive T lymphocytes decreases their activation threshold. These results identify FAM65B as a potential new target for controlling proliferation of both transformed and normal cells.


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