Oncotarget

Research Papers:

MALDI imaging reveals NCOA7 as a potential biomarker in oral squamous cell carcinoma arising from oral submucous fibrosis

Xiaoyan Xie, Yuchen Jiang, Yao Yuan, Peiqi Wang, Xinyi Li, Fangman Chen, Chongkui Sun, Hang Zhao, Xin Zeng, Lu Jiang, Yu Zhou, Hongxia Dan, Mingye Feng, Rui Liu _ and Qianming Chen

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Oncotarget. 2016; 7:59987-60004. https://doi.org/10.18632/oncotarget.11046

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Abstract

Xiaoyan Xie1,*, Yuchen Jiang1,*, Yao Yuan1,*, Peiqi Wang1, Xinyi Li1, Fangman Chen1, Chongkui Sun1, Hang Zhao1, Xin Zeng1, Lu Jiang1, Yu Zhou1, Hongxia Dan1, Mingye Feng1, Rui Liu1, Qianming Chen1

1State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China

*These authors have contributed equally to this work

Correspondence to:

Rui Liu, email: liurui_scu@hotmail.com

Qianming Chen, email: qmchen@scu.edu.cn

Keywords: NCOA7, MALDI Imaging, oral squamous cell carcinoma, oral submucous fibrosis, aryl hydrocarbon receptor

Received: December 14, 2015     Accepted: June 09, 2016     Published: August 4, 2016

ABSTRACT

Oral squamous cell carcinoma (OSCC) ranks among the most common cancer worldwide, and is associated with severe morbidity and high mortality. Oral submucous fibrosis (OSF), characterized by fibrosis of the mucosa of the upper digestive tract, is a pre-malignant lesion, but the molecular mechanisms underlying this malignant transformation remains to be elucidated. In this study, matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS)-based proteomic strategy was employed to profile the differentially expressed peptides/proteins between OSCC tissues and the corresponding adjacent non-cancerous OSF tissues. Sixty-five unique peptide peaks and nine proteins were identified with altered expression levels. Of them, expression of NCOA7 was found to be up-regulated in OSCC tissues by immunohistochemistry staining and western blotting, and correlated with a pan of clinicopathologic parameters, including lesion site, tumor differentiation status and lymph node metastasis. Further, we show that overexpression of NCOA7 promotes OSCC cell proliferation in either in vitro or in vivo models. Mechanistic study demonstrates that NCOA7 induces OSCC cell proliferation probably by activating aryl hydrocarbon receptor (AHR). The present study suggests that NCOA7 is a potential biomarker for early diagnosis of OSF malignant transformation, and leads to a better understanding of the molecular mechanisms responsible for OSCC development.


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