Oncotarget

Research Papers: Pathology:

Epsin2 promotes polarity establishment and meiotic division through activating Cdc42 in mouse oocyte

Ling Li, Longsen Han, Jiaqi Zhang, Xiaohui Liu, Rujun Ma, Xiaojing Hou, Juan Ge and Qiang Wang _

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Oncotarget. 2016; 7:50927-50936. https://doi.org/10.18632/oncotarget.10815

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Abstract

Ling Li1,*, Longsen Han1,*, Jiaqi Zhang1, Xiaohui Liu1,2, Rujun Ma3, Xiaojing Hou1, Juan Ge1 and Qiang Wang1

1 State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China

2 College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China

3 Center of Reproductive Medicine, Nanjing Jinling Hospital, Nanjing University School of Medicine, Nanjing, China

* These authors have contributed equally to this work

Correspondence to:

Qiang Wang, email:

Keywords: oocyte, meiosis, cytoskeleton, polarity, Epsin2, Pathology Section

Received: June 27, 2016 Accepted: July 14, 2016 Published: July 24, 2016

Abstract

Epsins are a conserved family of endocytic adaptors essential for diverse biological events. However, its role in oocytes remains completely unknown. Here, we report that specific depletion of Epsin2 in mouse oocytes significantly disrupts meiotic progression. Confocal microscopy reveals that Epsin2 knockdown results in the failure of actin cap formation and polar body extrusion during meiosis, indicative of the importance of Epsin2 in polarity establishment and cytokinesis. In addition, spindle defects and chromosome misalignment are readily observed in oocytes depleted of Epsin2. Moreover, we find that Epsin2 knockdown markedly decreases the activity of Cdc42 in oocytes and importantly, that the dominant-positive mutant of Cdc42 (Cdc42Q61L) is capable of partially rescuing the deficient phenotypes of Epsin2-knockdown oocytes. Together, our data identify Epsin2 as a novel player in regulating oocyte maturation, and demonstrate that Epsin2 promotes polarity establishment and meiotic division via activating Cdc42.


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