Oncotarget

Research Papers:

Tristetraprolin functions in cytoskeletal organization during mouse oocyte maturation

Xiaohui Liu, Xiaoyan Li, Rujun Ma, Bo Xiong, Shao-Chen Sun, Honglin Liu and Ling Gu _

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Oncotarget. 2016; 7:53330-53338. https://doi.org/10.18632/oncotarget.10755

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Abstract

Xiaohui Liu1, Xiaoyan Li1, Rujun Ma2, Bo Xiong1, Shao-Chen Sun1, Honglin Liu1, Ling Gu1

1College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China

2Center of Reproductive Medicine, Jinling Hospital, Nanjing, China

Correspondence to:

Ling Gu, email: lgu@njau.edu.cn

Keywords: oocyte, meiosis, cytoskeleton, reproduction, TTP

Received: April 22, 2016     Accepted: July 10, 2016     Published: July 21, 2016

ABSTRACT

Tristetraprolin (TTP), a member of TIS11 family containing CCCH tandem zinc finger, is one of the best characterized RNA-binding proteins. However, to date, the role of TTP in mammalian oocytes remains completely unknown. In the present study, we report the altered maturational progression and cytokinesis, upon specific knockdown of TTP in mouse oocytes. Furthermore, by confocal scanning, we observe the failure to form cortical actin cap during meiosis of TTP-depleted oocytes. Loss of TTP in oocytes also results in disruption of meiotic spindle morphology and chromosome alignment. In support of these findings, incidence of aneuploidy is accordingly increased when TTP is abated in oocytes. Our results suggest that TTP as a novel cytoskeletal regulator is required for spindle morphology/chromosome alignment and actin polymerization in oocytes.


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