Oncotarget

Research Papers:

Decreased Dp71 expression is associated with gastric adenocarcinoma prognosis

Sipin Tan _, Jin Tan, Sichuang Tan, Shuai Zhao, Xiaoxia Cao, Zhikang Chen, Qiaocheng Weng, Huali Zhang, Kangkai Wang, Jiang Zhou and Xianzhong Xiao

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Oncotarget. 2016; 7:53702-53711. https://doi.org/10.18632/oncotarget.10724

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Abstract

Sipin Tan1, Jin Tan1, Sichuang Tan2, Shuai Zhao1, Xiaoxia Cao1, Zhikang Chen3, Qiaocheng Weng3, Huali Zhang1, Kangkai Wang1, Jiang Zhou1, Xianzhong Xiao1

1Laboratory of Shock, Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha, Hunan 410008, People’s Republic of China

2Department of Thoracic Surgery, Second Xiangya Hospital, Central South University, Hunan 410011, People’s Republic of China

3Department of General Surgery, Xiangya Hospital, Central South University, Changsha 410008, People’s Republic of China

Correspondence to:

Sipin Tan, email: springtan@csu.edu.cn

Xianzhong Xiao, email: xiaoxianzhong@csu.edu.cn

Keywords: Dp71, lamin B1, gastric cancer, gastric cancer cells, prognosis

Received: November 24, 2015    Accepted: July 06, 2016    Published: July 20, 2016

ABSTRACT

For the first time, dramatically decreased Dp71 protein and mRNA was found in 34 pairs of resected primary gastric adenocarcinoma. Immunohistochemistry identified Dp71 expression suppressed in 72.2% of 104 gastric cancer patients. The decreased Dp71 expression was significantly correlated with cancer differentiation (P=0.001) and lymph vascular invasion (p=0.041). Decreased Dp71 expression was associated with a poor gastric adenocarcinoma prognosis (P=0.001). Significantly less Dp71 mRNA and protein were found in BGC823, SGC7901, AGS compared with GES-1. Via increasing lamin B1 mRNA and protein, enforced Dp71d and Dp71f expression resulted in SGC7901 proliferation inhibition. Co-IP proved interaction of Dp71 with lamin B1 in GES-1 cells. Further expression characterization showed reduced lamin B1 in gastric cancer tissue and cancer cells. Increasing lamin B1 expression results in the growth inhibition of SGC7901, which suggests that Dp71-lamin B1 protein complex plays an important role for the newly identified tumor suppressive function of Dp71.


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