Oncotarget

Research Papers:

Attribution of NKG2DL to the inhibition of early stage allogeneic tumors in mice

Li Hua, Mingli Fang, Boqi Dong, Sheng Guo, Cuiyun Cui, Jiwei Liu, Yun Yao, Yue Xiao, Xin Li, Yunjia Ren, Xiuping Meng, Xu Hao, Peiyan Zhao, Yilan Song, Liying Wang _ and Yongli Yu

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Oncotarget. 2016; 7:82369-82383. https://doi.org/10.18632/oncotarget.10693

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Abstract

Li Hua1, Mingli Fang2, Boqi Dong1, Sheng Guo2, Cuiyun Cui1, Jiwei Liu2, Yun Yao2, Yue Xiao2, Xin Li2, Yunjia Ren2, Xiuping Meng1, Xu Hao1, Peiyan Zhao2, Yilan Song1, Liying Wang2, Yongli Yu1

1Department of Immunology, College of Basic Medical Sciences, Norman Bethune Health Science Center, Jilin University, Changchun, Jilin 130021, China

2Department of Molecular Biology, College of Basic Medical Sciences, Norman Bethune Health Science Center, Jilin University, Changchun, Jilin 130021, China

Correspondence to:

Liying Wang, email: wlying@jlu.edu.cn

Yongli Yu, email: ylyu@jlu.edu.cn

Keywords: NKG2DL, allogeneic tumors, early stage, NK cells, NKG2D+ cells

Received: October 12, 2015     Accepted: June 09, 2016     Published: July 19, 2016

ABSTRACT

Allogeneic tumors are eventually rejected by adaptive immune responses, however, little is known about how allogeneic tumors are eradicated at the early stage of tumor development. In present study, we found that NKG2DL low expressing cancer cells were developed into palpable allogeneic tumors in mice within a week after the inoculation, while NKG2DL high expressing cancer cells failed to. The NKG2DL high expressing cancer cells could increase NKG2D+ NK cells in the allogeneic mice after being inoculated for 3 days. Artificially up-regulating NKG2DL on cancer cells with low level expressed NKG2DL by a CpG ODN resulted in the retardation and rejection of the allogeneic tumors at the early stage. The contribution of up-regulated NKG2DL to the early rejection was further confirmed by the results that the development of allogeneic tumors from cancer cells transfected with NKG2DL genes was significantly inhibited in mice at the early stage. Overall, hopefully, the data may provide insights for combining the allogeneic NK cell adoptive transfer with the approaches of up-regulating NKG2DL to treat cancer patients.


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