FNDC3B promotes cell migration and tumor metastasis in hepatocellular carcinoma
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Chin-Hui Lin1, Yao-Wen Lin1, Ying-Chun Chen1, Chen-Chung Liao2, Yuh-Shan Jou3, Ming-Ta Hsu1, Chian-Feng Chen1
1VYM Genome Research Center, National Yang-Ming University, Taipei, Taiwan
2Proteomics Research Center, National Yang-Ming University, Taipei, Taiwan
3Institutes of Biomedical Sciences, Academia Sinica, Taipei, Taiwan
Chian-Feng Chen, email: email@example.com
Keywords: FNDC3B, hepatocellular carcinoma, metastasis
Received: April 05, 2016 Accepted: June 13, 2016 Published: July 01, 2016
Recurrence and metastasis are common in hepatocellular carcinoma (HCC) and correlate with poor prognosis. We investigated the role of fibronectin type III domain containing 3B (FNDC3B) in HCC metastasis. Overexpression of FNDC3B in HCC cell lines enhanced cell migration and invasion. On the other hand, knockdown of FNDC3B using short-hairpin RNA reduced tumor nodule formation in both intra- and extra-hepatic metastasis. High levels of FNDC3B were observed in metastatic HCCs and correlated with poor patient survival and shorter recurrence time. Mutagenesis and LC-MS/MS analyses showed that FNDC3B promotes cell migration by cooperating with annexin A2 (ANXA2). Furthermore, FNDC3B and ANXA2 expression correlated negatively with patient survival. Our results indicate that FNDC3B behaves like an oncogene by promoting cell migration. This suggests FNDC3B could serve as a biomarker and therapeutic target for HCC metastasis.
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