miR-200b is a key regulator of tumor progression and metabolism targeting lactate dehydrogenase A in human malignant glioma
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Su Hu1,*, Qian Jiang2,*, Dongdong Luo1, Lei Zhao3, Xin Fu2, Yuqin Chen2, Xue Song4, Lihua Li1, Hailin Zhao1, Yingfang He1, Biao Peng1
1Department of Neurosurgery, Cancer Center of Guangzhou Medical University, Guangzhou, Guangdong, 510095, China
2State Key Laboratory of Respiratory Disease, The 1st Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, 510120, China
3Department of Physiology, School of Basic Science, Guangzhou Medical University, Guangzhou, Guangdong, 510182, China
4Guangdong Provincial Hospital of TCM Breast Department, Guangzhou, Guangdong, 510006, China
*These authors have contributed equally to this work
Biao Peng, email: [email protected]
Keywords: glioma, miR-200b, LDHA, proliferation, metabolism
Received: December 04, 2015 Accepted: June 04, 2016 Published: June 27, 2016
Lactate dehydrogenase A (LDHA) is involved in various cancers. In this study, we investigated the expression and function of LDHA in glioma. We found that LDHA was up-regulated in glioma samples. Furthermore, we found that overexpression of LDHA promoted proliferation, invasion and glycolysis in glioma cells. Luciferase reporter assays confirmed that LDHA was a direct target of miR-200b. miR-200b was found to be down-regulated in glioma samples, which was inversely correlated with LDHA expression. Repression of LDHA by miR-200b suppressed the glycolysis, cell proliferation and invasion of glioma cells. These results provide evidence that miR-200b acts as a tumor suppressor in glioma through the inhibition of LDHA both in vitro and in vivo. Targeting LDHA through miR-200b could be a potential therapeutic strategy in glioma.
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