Oncotarget

Research Papers:

Basal autophagy is pivotal for Hodgkin and Reed-Sternberg cells’ survival and growth revealing a new strategy for Hodgkin lymphoma treatment

Katrin Birkenmeier _, Katharina Moll, Sebastian Newrzela, Sylvia Hartmann, Stefan Dröse and Martin-Leo Hansmann

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Oncotarget. 2016; 7:46579-46588. https://doi.org/10.18632/oncotarget.10300

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Abstract

Katrin Birkenmeier1, Katharina Moll1, Sebastian Newrzela1, Sylvia Hartmann1, Stefan Dröse2, Martin-Leo Hansmann1

1Dr. Senckenberg Institute of Pathology, University Hospital of Frankfurt, 60596 Frankfurt am Main, Germany

2Clinic of Anesthesiology, Intensive-Care Medicine and Pain Therapy, Goethe-University Hospital, 60596 Frankfurt am Main, Germany

Correspondence to:

Katrin Birkenmeier, email: birkenmeier@em.uni-frankfurt.de

Keywords: B-cell lymphoma, Hodgkin lymphoma, autophagy, lymphoma pathogenesis, targeted therapy

Received: February 1, 2016     Accepted: May 19, 2016     Published: June 27, 2016

ABSTRACT

As current classical Hodgkin lymphoma (cHL) treatment strategies have pronounced side-effects, specific inhibition of signaling pathways may offer novel strategies in cHL therapy. Basal autophagy, a regulated catabolic pathway to degrade cell’s own components, is in cancer linked with both, tumor suppression or promotion. The finding that basal autophagy enhances tumor cell survival would thus lead to immediately testable strategies for novel therapies. Thus, we studied its contribution in cHL.

We found constitutive activation of autophagy in cHL cell lines and primary tissue. The expression of key autophagy-relevant proteins (e.g. Beclin-1, ULK1) and LC3 processing was increased in cHL cells, even in lymphoma cases. Consistently, cHL cells exhibited elevated numbers of autophagic vacuoles and intact autophagic flux. Autophagy inhibition with chloroquine or inactivation of ATG5 induced apoptosis and reduced proliferation of cHL cells. Chloroquine-mediated inhibition of basal autophagy significantly impaired HL growth in-vivo in NOD SCID γc-/- (NSG) mice. We found that basal autophagy plays a pivotal role in sustaining mitochondrial function.

We conclude that cHL cells require basal autophagy for growth, survival and sustained metabolism making them sensitive to autophagy inhibition. This suggests basal autophagy as useful target for new strategies in cHL treatment.


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